Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri
Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri
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DOI：
10.1016/j.cryobiol.2005.11.002
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发表时间：
2006-04-01
期刊：
影响因子：
2.7
通讯作者：
Tiersch, TR
中科院分区：
文献类型：
--
作者：
Yang, HP;Hazelwood, L;Tiersch, TR
本研究对剑尾剑尾鱼精子的冷藏和冷冻保存进行了研究。以往对剑鱼的冷冻保存研究都使用了活动精子，因为与大多数鱼类不同，剑鱼的精子在收集后冷藏一周后仍能保持持续的活动。然而，该物种通过内部受精繁殖，考虑到雌性生殖道内运动能力的显著要求和精子能量能力的潜在限制，在受精前固定精子可以提高受精成功率。因此，本研究的目的是利用渗透压来抑制海参采集后精子的运动能力，并测试固定对冷藏和冷冻保存的影响。目的是：(1)估计精子在不同渗透压下的活动性，并确定适合固定的渗透压；(2)冷冻保存固定的精子，并评估解冻或不稀释后的活力；(3)比较解冻后未固定和固定的精子的活力，离心。，并清洗以去除冷冻保护剂。将精子悬浮在11种不同渗透压（24-500 mOsmol/kg）的汉克斯平衡盐溶液（HBSS）中，测定精子的运动能力。活力在116 ~ 425 mOsmol/kg之间。当渗透压低于116 mmol /kg或高于425 mmol /kg时，精子不活动。当渗透压为291 ~ 316mosmol /kg时，可激活固定（无运动）精子的活力，且高渗HBSS （>= 425mOsmol/kg）固定精子的活力显著高于低渗HBSS (
In this study, refrigerated storage and cryopreservation of sperm from the green swordtail Xiphophorus helleri were investigated. Previous cryopreservation research in this species utilized motile sperm because unlike in most fish species, Xiphophorus sperm can remain continuously motile after collection for it week with refrigerated storage. However, this species reproduces by internal fertilization, and given the significant requirements For motility within the female reproductive tract and potential limitations on sperm energetic capacities, immobilization of sperm prior to insemination could be used to improve fertilization success. Thus, the goal in this Study was to use osmotic pressure to inhibit the motility of sperm after collection from X. helleri, and to test the effect of immobilization on refrigerated storage and cryopreservation. The objectives were to: (1) estimate the motility of sperm at different Osmotic pressures, and determine an osmotic pressure suitable for immobilization; (2) cryopreserve the immobilized sperm, and estimate the motility after thawing with Or Without dilution, and (3) compare motility of non-immobilized and immobilized sperm after thawing, Centrifugation., and washing to remove cryoprotectant. Motility was determined when sperm were suspended in 11 different osmotic pressures (24-500 mOsmol/kg) of Hanks' balanced salt solution (HBSS). Motility was observed between 116 and 425 mOsmol/kg. Sperm were not motile when the osmolality was lower than 116 or higher than 425 mOsmol/kg. Motility of the immobilized (nonmotile) sperm could be activated by changing the osmotic pressure to 291-316 mOsmol/kg, and motility of immobilized sperm from hypertonic HBSS (>= 425mOsmol/kg) was significantly higher than that from hypotonic HBSS (