Capturing change in clonal composition amongst single mouse germinal centers.
Capturing change in clonal composition amongst single mouse germinal centers.
复制标题
捕获单小鼠生发中心之间克隆组成的变化。
DOI：
10.7554/elife.33051
复制
发表时间：
2018-08-01
期刊：
影响因子：
7.7
通讯作者：
Carroll MC
中科院分区：
文献类型：
--
作者：
Firl DJ;Degn SE;Padera T;Carroll MC
要了解在几天到几周的时间尺度上体内发生的细胞过程，需要在不扰乱体内平衡的情况下反复询问同一组织。我们描述了一种新的设置纵向活体成像小鼠外周淋巴结（LNs）。在数天至数周内观察到单个生发中心（GC）的形成和演变。幼稚B细胞遇到抗原并形成原发灶，随后接种GC。这些经历了广泛变化的速度的选择，即使在紧密限制的空间接近。GC的流动性比以前观察到的更大，在短时间尺度上克隆性发生了很大的变化; GC的丢失是一种罕见的可观察到的事件。在同一动物内GC之间的克隆组成的同时、一致的变化的观察表明记忆B细胞的GC间运输。该工具改进了解决外周淋巴结中免疫动力学的方法，具有高时空分辨率和最小的稳态扰动。
Understanding cellular processes occurring in vivo on time scales of days to weeks requires repeatedly interrogating the same tissue without perturbing homeostasis. We describe a novel setup for longitudinal intravital imaging of murine peripheral lymph nodes (LNs). The formation and evolution of single germinal centers (GCs) was visualized over days to weeks. Naïve B cells encounter antigen and form primary foci, which subsequently seed GCs. These experience widely varying rates of homogenizing selection, even within closely confined spatial proximity. The fluidity of GCs is greater than previously observed with large shifts in clonality over short time scales; and loss of GCs is a rare, observable event. The observation of contemporaneous, congruent shifts in clonal composition between GCs within the same animal suggests inter-GC trafficking of memory B cells. This tool refines approaches to resolving immune dynamics in peripheral LNs with high temporospatial resolution and minimal perturbation of homeostasis.