Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging
Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging
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DOI：
10.1523/jneurosci.3727-03.2004
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发表时间：
2004-07-21
影响因子：
5.3
通讯作者：
Zhong, Y
中科院分区：
文献类型：
--
作者：
Wang, YL;Guo, HF;Zhong, Y
为了研究嗅觉信息在高级脑中枢的表达，我们在果蝇蘑菇体（MB）中表达了一种基于绿色荧光蛋白的钙离子传感器G-CaMP。使用双光子显微镜，我们成像气味诱发的G-CaMP荧光瞬变MB神经元[凯尼恩细胞（KC）]与单细胞分辨率。气味产生大的荧光瞬变的KC胞体的一个子集，并在限制性地区的花萼，MB的neurological。在不同的KC中，气味诱发的荧光瞬变表现出不同的变化与气味浓度：在一些KC中，荧光瞬变引起的气味浓度跨越几个数量级，而在其他人只有在一个狭窄的浓度范围。不同的气味产生荧光瞬变在不同的子集的KC。显示由给定气味引起的荧光瞬变的KC的空间分布在个体之间是相似的。对于某些气味，个别KC与特定的气味引起的荧光瞬变可以发现在类似的位置在不同的苍蝇的KC胞体的大小的顺序上的空间精度。这些结果表明，气味诱发的活动可以在MB中具有显着的空间特异性。
To study the representation of olfactory information in higher brain centers, we expressed a green fluorescent protein-based Ca2+ sensor, G-CaMP, in the Drosophila mushroom body (MB). Using two-photon microscopy, we imaged odor-evoked G-CaMP fluorescence transients in MB neurons [Kenyon cells (KCs)] with single-cell resolution. Odors produced large fluorescence transients in a subset of KC somata and in restricted regions of the calyx, the neuropil of the MB. In different KCs, odor-evoked fluorescence transients showed diverse changes with odor concentration: in some KCs, fluorescence transients were evoked by an odor at concentrations spanning several orders of magnitude, whereas in others only at a narrow concentration range. Different odors produced fluorescence transients in different subsets of KCs. The spatial distributions of KCs showing fluorescence transients evoked by a given odor were similar across individuals. For some odors, individual KCs with fluorescence transients evoked by a particular odor could be found in similar locations in different flies with spatial precisions on the order of the size of KC somata. These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB.