STIMULATORY ROLE OF GLIAL CELLS ON THE DIFFERENTIATION OF DISSOCIATED CHICK-EMBRYO ENCEPHALON CELLS
STIMULATORY ROLE OF GLIAL CELLS ON THE DIFFERENTIATION OF DISSOCIATED CHICK-EMBRYO ENCEPHALON CELLS
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DOI：
10.1159/000112568
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
SENSENBRENNER, M
中科院分区：
文献类型：
--
作者：
TOUZET, N;SENSENBRENNER, M
将孵育36 h的鸡胚脑分离细胞培养在成纤维细胞、脑膜细胞或神经胶质层上。在培养的第一个24小时内，细胞粘附在预制层上比粘附在塑料或胶原表面上更牢固。培养48小时后，脑膜细胞或成纤维细胞层上培养的脑细胞的行为非常类似于塑料表面上培养的那些细胞。当在神经胶质层上培养3天时，脑细胞分化成特征性的成神经细胞，如通过特异性神经元染色所证明的。这些结果支持神经胶质细胞在神经元分化中的刺激作用的假设。这种刺激作用的可能原因进行了讨论。
Dissociated cells from chick embryo encephalon of 36 h of incubation were cultivated on fibroblast, meningeal cell, or glial layer. Within the first 24 of cultivation, cells adhered to the performed layers more firmly than to plastic or collagen surfaces. After 48 h of cultivation, the brain cells cultured on meningeal cell or fibroblast layers behaved very similar to those cells cultured on plastic surfaces. When cultured on glial layers for 3 days, the brain cells differentiated into characteristic neuroblasts, as demonstrated by specific neuronal stainings. These results support the hypothesis of a stimulatory role of glial cells in neuronal differentiation. The possible causes of this stimulatory action are discussed.