Apatite Containing Aspartic Acid for Selective Protein Loading
Apatite Containing Aspartic Acid for Selective Protein Loading
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DOI：
10.1177/0022034509357309
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发表时间：
2010-05-01
影响因子：
7.6
通讯作者：
Sohmura, T.
中科院分区：
文献类型：
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作者：
Uddin, M. Hafiz;Matsumoto, T.;Sohmura, T.
羟基磷灰石(HAP)材料的物理化学修饰被认为是开发新型生物活性载体材料用于药物输送和组织工程应用的先决条件。由于酸性氨基酸对HAP和碱性蛋白质都有亲和力，天冬氨酸(Asp)修饰的HAP可能是碱性蛋白质载体的候选底物之一。在这里，我们在不同浓度的天冬氨酸存在下合成了羟基磷灰石，观察到羟基磷灰石的结晶度和其他物理化学性质得到了有效的调节。详细的研究表明，天冬氨酸没有被引入到HAP晶格中，而是被捕获在HAP晶体中。蛋白质吸附研究表明，天冬氨酸修饰的羟基磷灰石颗粒对碱性蛋白质具有选择性负载能力。因此，含有天冬氨酸的羟基磷灰石颗粒在药物传递方面具有潜在的应用前景，特别是作为碱性蛋白的载体，包括碱性成纤维细胞生长因子和骨形成蛋白。
Physico-chemical modifications of hydroxyapatite (HAp) materials are considered as pre-requisites for the development of new bioactive carrier materials for drug delivery and tissue engineering applications. Since acidic amino acids have well-documented affinities to both HAp and basic proteins, HAp modified by aspartic acid (Asp, acidic amino acid) might be one of the candidate substrates for a basic protein carrier. Here, we synthesized HAp in the presence of various concentrations of Asp and observed that HAp crystallinity and other physico-chemical properties were effectively modulated. Detailed studies indicated that Asp was not incorporated in the HAp crystal lattice, but rather was trapped in HAp crystals. Protein adsorption studies indicated that the HAp particles modified by Asp had a selective loading capacity for basic protein. Therefore, HAp particles containing Asp might have potential in drug delivery applications, especially as the carrier of basic proteins including bFGF and BMP.