Inhibitory effect of monoclonal antibodies on the growth of Babesia caballi
Inhibitory effect of monoclonal antibodies on the growth of Babesia caballi
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DOI：
10.1016/s0020-7519(99)00137-x
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发表时间：
1999-11-01
影响因子：
4
通讯作者：
Mikami, T
中科院分区：
文献类型：
--
作者：
Ikadai, H;Tamaki, Y;Mikami, T
制备了针对caballi巴贝虫（USDA菌株）的单克隆抗体（mab），以确定用于诊断和疫苗开发的种特异性抗原。选择间接免疫荧光抗体检测的8个caballi单抗阳性克隆进行纯化和进一步鉴定。激光共聚焦显微镜观察发现，单克隆抗体识别的抗原分别位于caballi寄生虫的表面/细胞质、中央部分和/或前端，具有5种不同的反应模式。这些单抗似乎是种特异性的，因为它们不会与巴贝斯虫感染的红细胞或未感染的红细胞发生交叉反应。在Western blotting分析中，6种不同的单抗分别识别了caballi裂殖子的18、20、34、36、48和155 kDa蛋白。当添加到体外培养时，其中4个单抗显著抑制caballi寄生虫的体外生长。这些结果为评估抗原以开发诊断方法或疫苗提供了依据。(C) 1999澳大利亚寄生虫学学会Elsevier Science Ltd.出版。版权所有。
Monoclonal antibodies (mAbs) were produced against Babesia caballi (USDA strain) to define a species-specific antigen for use in diagnosis and vaccine development. Eight positive clones of B. caballi mAbs determined by indirect immunofluorescent antibody test were selected for purification and further characterisation. Confocal laser microscopy showed that the antigens recognised by the mAbs were located on the surface/cytoplasm, central part, and/or anterior end of B. caballi parasites, with five different reactive patterns. These mAbs seemed to be species-specific, since they did not cross-react with Babesia equi-infected erythrocytes or uninfected erythrocytes. In Western blotting analysis, 18, 20, 34, 36, 48, and 155 kDa proteins of B. caballi merozoites were recognised by six different mAbs. When added to in vitro cultures, four of the mAbs significantly inhibited the in vitro growth of B. caballi parasites. These results provide a rationale for evaluating antigens for the development of diagnostic methods or vaccines. (C) 1999 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.