Molecular Epidemiological Characterization of Methicillin-Susceptible and -Resistant Staphylococcus aureus Isolated from Skin and Soft Tissue Infections in Bangladesh
Molecular Epidemiological Characterization of Methicillin-Susceptible and -Resistant Staphylococcus aureus Isolated from Skin and Soft Tissue Infections in Bangladesh
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DOI：
10.1089/mdr.2018.0123
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发表时间：
2019-03-01
影响因子：
2.6
通讯作者：
Kobayashi, Nobumichi
中科院分区：
文献类型：
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作者：
Haque, Nazia;Aung, Meiji Soe;Kobayashi, Nobumichi
2015年至2016年在孟加拉国中北部地区研究了从皮肤和软组织感染患者中收集的金黄色葡萄球菌的遗传背景和分子特征。在430株临床分离的耐甲氧西林链球菌中，以耐甲氧西林链球菌为主。金黄色葡萄球菌（MRSA）中SCCmec IV型占73%、V型占14%（31%），主要为凝固酶（coa）基因型IIa、IIIa、IVb和XIa，而甲氧西林敏感的S.金黄色葡萄球菌（MSSA）的最高峰为IIIa，其次为Va、IIa和VIa。Panton-Valentine杀白细胞素基因（pvl）在MSSA中的检出率（54%）高于MRSA（24%）。根据多位点序列分型，pvl阳性MRSA分离株分为克隆复合物88（CC 88）（ST 88、ST 2884、ST 4345）、CC 6（ST 6、ST 4350）和CC 1（ST 1、ST 772），而pvl阴性MRSA分为CC 5、CC 22、CC 80、CC 121和CC 672。pvl阴性ST 80 MRSA分离株具有SCCmec-IVa（agr-III/coa-XIc，etd/edinB阳性，fusB阴性），表明它们属于与欧洲共同体获得的MRSA克隆相关的新CC 80分支。在MSSA中，在pvl阳性和阴性分离株中鉴定出ST 121/spa-t645/coa-Va和ST 2884（CC 88）/spa-t2393/coa-IIIa基因型，并且所有ST 772分离株均携带pvl。所有的ST 121菌株都有一个弹性蛋白结合蛋白基因（ebpS-v）的变异体，内部180个核苷酸的缺失。本研究表明，CC 88（ST 88，ST 2884）和ST 772是pvl阳性MRSA/MSSA的推定优势谱系，而新的CC 80分支是孟加拉国地方分布的主要pvl阴性MRSA谱系之一。
Genetic background and molecular characteristics of Staphylococcus aureus collected from patients with skin and soft tissue infections were studied in the North-Central region of Bangladesh from 2015 to 2016. Among 430 clinical isolates, methicillin-resistant S. aureus (MRSA) accounted for 31% having SCCmec type IV (73%) and V (14%), and belonged mostly to coagulase (coa) genotypes IIa, IIIa, IVb, and XIa, while dominant coa type in methicillin-susceptible S. aureus (MSSA) was IIIa, followed by Va, IIa, and VIa. Panton-Valentine Leukocidin genes (pvl) were detected at higher rate in MSSA (54%) than in MRSA (24%). Based on multilocus sequence typing, pvl-positive MRSA isolates were classified into clonal complex 88 (CC88) (ST88, ST2884, ST4345), CC6 (ST6, ST4350), and CC1 (ST1, ST772), while pvl-negative MRSA into CC5, CC22, CC80, CC121, and CC672. The pvl-negative ST80 MRSA isolates had SCCmec-IVa (agr-III/coa-XIc, etd/edinB-positive, fusB-negative), indicating that they belong to the novel CC80 clade related to the European community-acquired MRSA clone. Among MSSA, genotypes ST121/spa-t645/coa-Va and ST2884 (CC88)/spa-t2393/coa-IIIa were identified in both pvl-positive and negative isolates, and all the ST772 isolates harbored pvl. All the ST121 isolates had a variant of elastin-binding protein gene (ebpS-v) with internal 180-nucleotide deletion. The present study suggested that CC88 (ST88, ST2884) and ST772 are the putative dominant lineages of pvl-positive MRSA/MSSA, while novel CC80 clade is one of the main pvl-negative MRSA lineages distributed endemically in Bangladesh.