Specificity and suppressor function of human T cells responsive to autologous non-T cells.
Specificity and suppressor function of human T cells responsive to autologous non-T cells.
复制标题
人类 T 细胞对自体非 T 细胞反应的特异性和抑制功能。
作者：
T. Sakane;I. Green
人类T细胞对自体非T细胞的增殖反应。这种被称为自体MLR的反应已被证明具有免疫特异性和记忆性。我们目前的研究首先证明了在自体MLR中可以证明特异性的继发性反应。然后使用BUdR和光技术来消融对自体或异体非t细胞反应的增殖细胞。去除对自身非T细胞有反应的T细胞后，对异体T细胞的反应相对完整，反之，去除对异体非T细胞有反应的T细胞后，对自身非T细胞的反应相对完整。因此，对自体非T细胞有反应的T细胞和对异体非T细胞有反应的T细胞在很大程度上是不同的细胞群。
Human T cells proliferate in response to autologous non-T cells. This reaction, called the autologous MLR has been shown to have both immunologic specificity and memory. Our present study first demonstrates that specific secondary responses can be demonstrated in the autologous MLR. A BUdR and light technique was then used to ablate cells proliferating in response to either autologous or allogeneic non-T cells. The removal of T cells responsive to autologous non-T cells left the responsiveness to allogeneic T cells relatively intact, and conversely the removal of T cells responsive to allogeneic non-T cells left the responsiveness to autologous non-T cells relatively intact. Thus, the T cells responsive to autologous non-T cells and those responsive to allogeneic non-T cells are largely separate cell populations.
Since the lymphocytes from patients with SLE fail to demonstrate a normal autologous MLR and also have a defect in the development of Con A-induced suppressor cells, we investigated whether the T cell population responsive in the autologous MLR was particularly enriched in cells capable of developing suppressor capacity. A BUdR and light treatment was used to remove T cells responsive to either autologous or allogeneic non-T cells. The remaining cells were then activated by Con A, and their subsequent suppressor activity was measured. Removal of T cells proliferating in response to autologous non-T cells produced significantly greater failure of suppressor cell development than did the removal of T cells responsive to allogeneic non-T cells. Thus, T cells capable of responding to autologous non-T cells are particularly enriched in cells capable of becoming suppressor cells.