Oxytocin receptors in human uterus.
Oxytocin receptors in human uterus.
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人类子宫中的催产素受体。
DOI：
10.1210/jcem-38-6-1052
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
A. Steinberg
中科院分区：
文献类型：
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作者：
M. Soloff;T. Swartz;A. Steinberg
摘要[3 H]催产素（[3 H]OT）在体外与2名妊娠14-16周妇女子宫肌层制备的20，000 × g颗粒部分结合。两个子宫标本中催产素结合的表观解离常数分别为1.99 ± 0.18（se）× 10−9 m（n = 9）和2.81 ± 0.26 × 10−9 m（n = 10）。一类单一的结合位点是明显的OT浓度范围内检查，0.6至2.2 nm。每毫克颗粒蛋白质结合约1.8 × 10−13摩尔OT。合成的OT类似物对[~ 3 H]OT结合位点的竞争是特异的：[脱氨基]OT > [8-缬氨酸]OT>[4-苏氨酸]OT > OT > [8-赖氨酸]加压素>脱氨基托辛> [4-脯氨酸]OT。OT结合位点似乎是子宫受体的一部分，因为它们具有高亲和力和配体特异性。
ABSTRACT [3H]oxytocin ([3H]OT) was bound in vitro to the 20,000 × g particulate fraction prepared from the myometrium of 2 women, 14–16 weeks pregnant. The apparent dissociation constants for oxytocin binding in preparations of the two uteri were 1.99 ± 0.18 (se) × 10−9 m (n = 9) and 2.81 ± 0.26 × 10−9 m (n = 10), respectively. A single class of binding sites was apparent within the range of OT concentrations examined, 0.6 to 2.2 nm. About 1.8 × 10−13 moles of OT were bound per mg of particulate protein. The competition for [3H]OT binding sites by synthetic analogues of OT was specific: [desamino]OT > [8-valine]OT ⋝ [4-threonine]OT > OT > [8-lysine]vasopressin > desaminotocinol > [4-proline]OT. The OT binding sites appear to be part of the uterine receptor because of their high affinity and ligand specificity.