Selection for higher fertility reflects in the seminal fluid proteome of modern domestic chicken
Selection for higher fertility reflects in the seminal fluid proteome of modern domestic chicken
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DOI：
10.1016/j.cbd.2016.10.006
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发表时间：
2017-03-01
影响因子：
3
通讯作者：
Rodriguez-Martinez, Heriberto
中科院分区：
文献类型：
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作者：
Atikuzzaman, Mohammad;Sanz, Libia;Rodriguez-Martinez, Heriberto
现代家鸡（白色来航鸡，WL）的高产蛋力是由低产蛋力的祖先红原鸡（RJF）经过连续的性状选育而产生的。为了研究这种长期选择是否影响精液（SF）蛋白质组，进行了基于2DE电泳的蛋白质组学分析和免疫测定，以绘制RJF、WL和RJF/WL-L13的第9代高级互交系（AIL）中的SF蛋白/细胞因子，包括单个SF（n = 4，来自每个RJF、WL和AIL组）和来自每组15名男性的SF池，通过2DE分析以确定其组内差异程度（AIL、WL和RJF）变异性;分别是品种间特异性SF蛋白斑点强度的组间倍数变化的品种间比较分析。PCA清楚地强调了个体公鸡之间的明显组内相似性以及明显的组间变异性（例如RJF，WL和AIL之间），验证了池的使用以最大限度地减少混淆个体变异。蛋白质表达变化很大的精子活力，营养，运输和生存的女性，包括信号对免疫调节的过程。SF-蛋白主要是血清白蛋白和卵转铁蛋白。天冬氨酸氨基转移酶，膜联蛋白A5，琥珀酸合成酶，谷胱甘肽S-转移酶2和L-乳酸脱氢酶-A是RJF特异性的。甘油醛-3-磷酸脱氢酶对WL-SF具有特异性，而血管紧张素转换酶、γ-烯醇化酶、凝血因子IX、纤维蛋白原α链、血红蛋白亚基α-D、溶菌酶C、磷酸甘油酸激酶、Src-底物蛋白p85、微管蛋白和硫氧还蛋白对AIL具有特异性。与WL和AIL相比，RJF-SF含有更少的免疫系统过程蛋白和更低量的抗炎/免疫调节TGF-β 2，与RJF相比，WL和AIL具有低水平或缺乏促炎CXCL 10。祖先鸡和现代鸡的精液蛋白质组不同，明显富集了与精子在雌性和生育力中的免疫调节相关的蛋白质和肽。(C)2016 Elsevier Inc. All rights reserved.
The high egg-laying capacity of the modem domestic chicken (i.e. White Leghorn, WL) has arisen from the low egg-laying ancestor Red Junglefowl (RJF) via continuous trait selection and breeding. To investigate whether this long-term selection impacted the seminal fluid (SF)-proteome, 2DE electrophoresis-based proteomic analyses and immunoassays were conducted to map SF-proteins/cytokines in RJF, WL and a 9th generation Advanced Intercross Line (AIL) of RJF/WL-L13, including individual SF (n = 4, from each RJF, WL and AIL groups) and pools of the SF from 15 males of each group, analyzed by 2DE to determine their degree of intra-group (AIL, WL, and RJF) variability using Principal Component Analysis (PCA); respectively an inter-breed comparative analysis of intergroup fold change of specific SF protein spots intensity between breeds. The PCA clearly highlighted a clear intra-group similarity among individual roosters as well as a clear inter-group variability (e.g. between RJF, WL and AIL) validating the use of pools to minimize confounding individual variation. Protein expression varied considerably for processes related to sperm motility, nutrition, transport and survival in the female, including signaling towards immunomodulation. The major conserved SF-proteins were serum albumin and ovotransferrin. Aspartate aminotransferase, annexin A5, arginosuccinate synthase, glutathione S-transferase 2 and L-lactate dehydrogenase-A were RJF-specific. Glyceraldehyde-3-phosphate dehydrogenase appeared specific to the WL-SF while angiotensin-converting enzyme, gamma-enolase, coagulation factor IX, fibrinogen alpha-chain, hemoglobin subunit alpha-D, lysozyme C, phosphoglycerate kinase, Src-substrate protein p85, tubulins and thioredoxin were AIL-specific. The RJF-SF contained fewer immune system process proteins and lower amounts of the anti-inflammatory/immunomodulatory TGF-beta 2 compared to WL and AIL, which had low levels- or lacked pro inflammatory CXCL10 compared to RJF. The seminal fluid proteome differs between ancestor and modern chicken, with a clear enrichment of proteins and peptides related to immune-modulation for sperm survival in the female and fertility. (C) 2016 Elsevier Inc. All rights reserved.