Isolation and Characterization of Novel Lytic Bacteriophage vB_RsoP_BMB50 infecting Ralstonia solanacearum
Isolation and Characterization of Novel Lytic Bacteriophage vB_RsoP_BMB50 infecting Ralstonia solanacearum
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DOI：
10.1007/s00284-022-02940-3
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发表时间：
2022-07
影响因子：
2.6
通讯作者：
Kai Wang;Dawei Chen;Quanrong Liu;Pengfei Zhu;M. Sun;Donghai Peng
中科院分区：
文献类型：
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作者：
Kai Wang;Dawei Chen;Quanrong Liu;Pengfei Zhu;M. Sun;Donghai Peng
青枯菌是一种土传植物病原菌，在世界范围内可引起多种重要农作物的青枯病，造成巨大的经济损失。然而，目前对日益严重的青枯病缺乏有效、绿色、安全的防控措施。噬菌体被认为是一种潜在的细菌性枯萎病生物防治剂。虽然许多细菌感染了R.虽然已经分离出了青枯菌属的噬菌体，但这些噬菌体尚不足以应对青枯菌属细菌的多样性。青枯菌属在本研究中，一种新的裂解性噬菌体vB_RsoP_BMB50感染多个R.青枯菌（Solanacearum）是从中国大连番茄田分离到的一种病原菌。透射电镜和基因组学分析表明，vB_RsoP_BMB50属于冈病毒亚科、Autographiviridae科、尾病毒目，其DNA为双链DNA，全长43,665 bp，平均G+C含量为61.79%，含有53个开放阅读框（ORF）。这种新型噬菌体表现出大的爆发尺寸、高温稳定性（4-50 °C）和强pH耐受性（pH 5-10）。比较分析和系统发育分析表明，vB_RsoP_BMB50与GenBank数据库中的其它噬菌体序列相似性较低，代表一个新的罗尔斯通氏噬菌体属。vB_RsoP_BMB50具有裂解谱广、热稳定性高、耐pH能力强等特点，被认为是一种有效的青枯病生防菌候选菌株。青枯菌属
Ralstonia solanacearumis a soil-borne phytopathogen, and it can cause bacterial wilt disease in a variety of key crops around the world, thus resulting in enormous financial losses. However, there is a lack of effective, green, and safe prevention and control measures against increasingly devastating bacterial wilt disease. Bacteriophages (phages) are considered as potential biocontrol agents against bacterial wilt disease. Although many phages infectingR. solanacearumhave been isolated, so far, theseRalstoniaphages are still insufficient to deal with the diversity of the bacteria ofR. solanacearum. In this study, a novel lytic bacteriophage vB_RsoP_BMB50 infecting multipleR. solanacearumwas isolated from tomato fields in Dalian, China. Transmission electron microscopy and genomics analysis indicated that vB_RsoP_BMB50 belonged to the subfamily Okabevirinae,Autographiviridaefamily, and order Caudovirales, and it comprised a double-stranded DNA with a full length of 43,665 bp and a mean G+C content of 61.79%, containing 53 open reading frames (ORFs). This novel phage exhibited a large burst size, high temperature stability (4–50 °C), and strong pH tolerance (pH 5–10). Comparative analyses and phylogenetic analyses revealed that vB_RsoP_BMB50 represented a novelRalstoniaphage genus since it exhibited a low sequence similarity to other phages in the GenBank database. Due to its broad lytic spectrum, high thermal stability, and strong pH tolerance, vB_RsoP_BMB50 is considered as an effective candidate biocontrol agent against bacterial wilt disease caused byR. solanacearum.