THE NEOSTRIATAL MOSAIC - COMPARTMENTAL DISTRIBUTION OF CALCIUM-BINDING PROTEIN AND PARVALBUMIN IN THE BASAL GANGLIA OF THE RAT AND MONKEY
THE NEOSTRIATAL MOSAIC - COMPARTMENTAL DISTRIBUTION OF CALCIUM-BINDING PROTEIN AND PARVALBUMIN IN THE BASAL GANGLIA OF THE RAT AND MONKEY
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DOI：
10.1073/pnas.82.24.8780
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发表时间：
1985-12-01
影响因子：
11.1
通讯作者：
MILLER, JJ
中科院分区：
文献类型：
--
作者：
GERFEN, CR;BAIMBRIDGE, KG;MILLER, JJ
钙结合蛋白（CaBP）和小清蛋白是两种在脑中表达并在微摩尔范围内结合钙的蛋白质。用免疫组织化学方法检测了这两种蛋白在大鼠和恒河猴基底节的分布。在纹状体中，CaBP免疫反应性定位于纹状体黑质投射神经元的子集; CaBP阳性神经元分布在含有生长抑素免疫反应性纤维的区域中，而不是在含有致密μ C的互补区域中。阿片受体结合这些生物化学标记分别标记纹状体的基质和斑块隔室。先前的研究表明，纹状体基质神经元投射到黑质网状部，而纹状体斑片神经元投射到黑质网状部。与限制本地化的钙结合蛋白在基质投射神经元，如果限制的钙结合蛋白免疫反应传入纤维的网状部。CaBP还定位于黑质腹侧被盖区的多巴胺能神经元和少数非多巴胺能神经元。小清蛋白免疫反应性定位于黑质网状部神经元及其轴突的一个子集。在侧纹状体，一些中等大小的棘状中间神经元也是小白蛋白免疫反应。CaBP和小清蛋白在基底神经节中的不同分布进行了讨论，在细胞内的钙缓冲系统中，它们所包含的神经元的生理反应特性相关的可能的作用。
Calcium-binding protein (CaBP) and parvalbumin are two proteins that are expressed in brain and bind calcium in the micromolar range. The immunohistochemical distribution of these two proteins were examined in the basal ganglia of rats and rhesus monkeys. In the striatum, CaBP immunoreactivity is localized to a subset of striatonigral projections neurons; CaBP-positive neurons are distributed in areas containing somatostatin-immunoreactive fibers and not in the complementary areas containing dense .mu. opiate-receptor binding. These biochemical labels mark, respectively, the matrix and patch compartments of the striatum. Previous studies have shown that striatal matrix neurons project to the substantia nigra pars reticulata, whereas striatal patch neurons project to the substantia nigra pars compacta. Consistent with the restricted localization of CaBP in the matrix projection neurons if the confinement of CaBP-immunoreactive afferent fibers to the pars reticulata. CaBP is also localized to a portion of dopaminergic and a few nondopaminergic neurons in the substantia nigra pars compacta and in most dopaminergic neurons in the ventral tegmental area. Parvalbumin immunoreactivity is localized to a subset of substantia nigra pars reticulata neurons and their axons. In the lateral striatum, some medium-sized aspiny interneurons are also parvalbumin immunoreactive. The distinct distributions of CaBP and parvalbumin in the basal ganglia are discussed in terms of their possible roles in intracellular calcium buffer systems related to the physiologic response properties of the neurons in which they are contained.