Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice
Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice
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DOI：
10.1016/j.ijbiomac.2020.06.107
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发表时间：
2020-11-01
影响因子：
8.2
通讯作者：
Ji, Huijie
中科院分区：
文献类型：
--
作者：
Duan, Zhen;Zhang, Yang;Ji, Huijie
本研究旨在探讨磷酸化平菇多糖（PPOP）的基本结构特征，并研究PPOP对四氯化碳所致雄性昆明小鼠肝损伤的保护作用。磷酸化多糖是从平菇（POP）中提取的天然多糖制备的。通过FT-IR、ESEM光谱和刚果红测试对PPOP和POP的结构进行了表征。化学成分分析表明，PPOP主要由鼠李糖、半乳糖醛酸和木糖组成，摩尔比为0.10:1.98:1.00。结构分析表明PPOP具有多链结构，吸收峰为P=O和P-O-C。此外，动物实验表明，PPOP的抗肝损伤作用体现在降低血清中丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、碱性磷酸酶、总胆固醇、三月桂酸甘油酯、低密度脂蛋白胆固醇的水平，增加血液中高密度脂蛋白胆固醇和白蛋白的含量，降低丙二醛的含量，促进肝脏抗氧化酶的活性。 PPOP比POP表现出更强的保肝作用和体内抗氧化活性。基于上述生物学研究，最终结果表明PPOP可用于治疗化学性肝毒性。 (c) 2020 Elsevier B.V. 保留所有权利。
This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice. The phosphorylated polysaccharide was prepared from the natural polysaccharide extracted from Pleurotus ostreatus (POP). The structures of PPOP and POP were characterized by FT-IR, ESEM spectroscopy, and Congo red test. Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00. Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of P=O and P-O-C. Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver. PPOP exhibited stronger hepatoprotective effect and antioxidant activity in vivo than POP. The final results indicated that PPOP could be used in the treatment of chemical-induced hepatotoxicity based on the above biological research. (c) 2020 Elsevier B.V. All rights reserved.