Flow microfluorometric analysis of isolated Chinese hamster chromosomes.
Flow microfluorometric analysis of isolated Chinese hamster chromosomes.
复制标题
分离的中国仓鼠染色体的流式显微荧光分析。
DOI：
10.1016/0014-4827(75)90519-4
复制
发表时间：
1975
影响因子：
3.7
通讯作者：
L. Deaven
中科院分区：
文献类型：
--
作者：
E. Stubblefield;S. Cram;L. Deaven
分离的中国仓鼠染色体用溴化乙锭染色后，用流式显微荧光法进行分析。观察到对应于每个常染色体的预期DNA含量的峰。通过蔗糖梯度区带离心部分分离的染色体的检查验证了整个核型的各个峰中的染色体的身份。这些意见的意义，核型分析进行了讨论。
Isolated Chinese hamster chromosomes were analysed by flow microfluorometry after staining with ethidium bromide. Peaks corresponding to the expected DNA content of each of the autosomes were observed. Examination of chromosomes partially fractionated by zonal centrifugation on sucrose gradients verified the identity of the chromosomes in the various peaks of the whole karyotype. The significance of these observations to karyotype analysis is discussed.