Magnesium Transporter 5 plays an important role in Mg transport for male gametophyte development in Arabidopsis
Magnesium Transporter 5 plays an important role in Mg transport for male gametophyte development in Arabidopsis
复制标题
镁转运蛋白 5 在拟南芥雄配子体发育的镁转运中发挥重要作用
DOI：
10.1111/tpj.13054
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发表时间：
2015
期刊：
影响因子：
7.2
通讯作者：
Yang Zhong-Nan
中科院分区：
文献类型：
--
作者：
Xu Xiao-Feng;Wang Bo;Lou Yue;Han Wen-Jian;Lu Jie-Yang;Li Dan-Dan;Li Le-Gong;Zhu Jian;Yang Zhong-Nan
During anther development the male gametophyte develops inside the locule and the tapetal cells provide all nutrients for its development.Magnesium Transporter 5(MGT5) is a member of the MGT family and has dual functions of Mg export and import. Here, we show that male gametophyte mitosis and intine formation are defective in amgt5mutant. The transient expression ofGFP‐MGT5revealed that MGT5 is localized in the plasma membrane. These findings suggest that in the male gametophyte MGT5 plays a role in importing Mg from the locule and that Mg is essential for male gametophyte development. The expression ofMGT5in the knockoutABORTED MICROSPORES(AMS) mutant (AMS being an essential regulator of tapetum) is tremendously reduced. Chromatin immunoprecipitation and mobility shift assay experiments demonstrated that AMS can directly bind the promoter ofMGT5. An immunoelectron microscopy assay revealed that MGT5‐His is localized to the plasma membrane of the tapetum. These findings suggest that AMS directly regulates MGT5 in the tapetum and thus induces export of Mg into the locule. Themgt5plant exhibits severe male sterility while the expression ofMGT5under the tapetum‐specific promoterA9partly rescuedmgt5fertility.mgt5fertility was restored under high‐Mg conditions. These findings suggest that themgt5tapetum still has the ability to export Mg and that a sufficient supply of Mg from the tapetum can improve the importation of Mg in themgt5male gametophyte. Therefore, MGT5 plays an important role in Mg transport from the tapetum to the microspore.
