Downregulation of cytoskeletal muscle LIM protein by nitric oxide - Impact on cardiac myocyte hypertrophy
Downregulation of cytoskeletal muscle LIM protein by nitric oxide - Impact on cardiac myocyte hypertrophy
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DOI：
10.1161/01.cir.0000055319.94801.fc
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发表时间：
2003-03-18
期刊：
影响因子：
37.8
通讯作者：
Wollert, KC
中科院分区：
文献类型：
--
作者：
Heineke, J;Kempf, T;Wollert, KC
背景：在慢性心力衰竭中，一氧化氮合酶（NOS2）诱导型异构体的心肌表达增强，导致一氧化氮的持续产生。我们假设NO调节心肌细胞中基因的表达，这些基因在心脏肥大和衰竭的情况下可能具有重要的功能。方法和结果：cDNA表达阵列分析显示，一氧化氮供体SNAP抑制激动剂诱导的心肌细胞肥大，下调内皮素-1 （ET-1）刺激的新生大鼠心肌细胞中细胞骨架相关肌LIM蛋白（MLP）的表达。Northern blotting和免疫印迹实验证实了这一发现，并确定SNAP负性控制MLP mRNA (-49%， P
Background-In chronic heart failure, myocardial expression of the inducible isoform of nitric oxide (NO) synthase (NOS2) is enhanced, leading to a sustained production of NO. We postulated that NO modulates expression of genes in cardiac myocytes that may be functionally important in the context of cardiac hypertrophy and failure.Methods and Results-As revealed by cDNA expression array analyses, the NO donor SNAP, which has been shown previously to inhibit agonist-induced cardiac myocyte hypertrophy, downregulates expression of the cytoskeleton-associated muscle LIM protein (MLP) in endothelin-1 (ET-1)-stimulated neonatal rat cardiac myocytes. Northern blotting and immunoblotting experiments confirmed this finding and established that SNAP negatively controls MLP mRNA (-49%, P