Toxin-coupled MHC class I tetramers can specifically ablate autoreactive CD8+ T cells and delay diabetes in nonobese diabetic mice.
Toxin-coupled MHC class I tetramers can specifically ablate autoreactive CD8+ T cells and delay diabetes in nonobese diabetic mice.
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DOI：
10.4049/jimmunol.0903931
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发表时间：
2010-04-15
期刊：
影响因子：
--
通讯作者：
Hess PR
中科院分区：
文献类型：
--
作者：
Vincent BG;Young EF;Buntzman AS;Stevens R;Kepler TB;Tisch RM;Frelinger JA;Hess PR
There is compelling evidence that self reactive CD8+ T cells are a major factor in development and progression of Type 1 diabetes in animals and humans. Hence, great effort has been expended to define the specificity of autoimmune CD8+ T cells, and to alter their responses. Much work has focused on tolerization of T cells using proteins or peptides. A weakness in this approach is residual autoreactive T cells may be activated and exacerbate disease. In this report we use a novel approach - toxin coupled MHC class I tetramers. Used for some time to identify antigen specific cells, here we use that same property to delete the antigen specific cells. We show saporin coupled tetramers can delete IGRP reactive T cells in vitro and in vivo. Sequence analysis of TCRβ chains of IGRP+ cells reveals the repertoire complexity in the islets is markedly decreased as NOD mice age and significantly altered in toxic tetramer treated NOD mice. Further tetramer+ T cells in the islets are almost completely deleted and surprisingly loss of tetramer+ T cells in the islets is long lasting. Finally, we show deletion at 8 weeks of age of IGRP+ CD8+ T cells, but not DMK or InsB reactive cells, significantly delays diabetes in NOD mice.
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DOI：
10.1016/j.clim.2009.04.007
发表时间：
2009-08
期刊：
Clinical immunology (Orlando, Fla.)
影响因子：
--
作者：
Herold KC;Gitelman S;Greenbaum C;Puck J;Hagopian W;Gottlieb P;Sayre P;Bianchine P;Wong E;Seyfert-Margolis V;Bourcier K;Bluestone JA;Immune Tolerance Network ITN007AI Study Group
通讯作者：
Immune Tolerance Network ITN007AI Study Group
影响因子：
4.4
作者：
Lieberman, SM;Takaki, T;DiLorenzo, TP
通讯作者：
DiLorenzo, TP
DOI：
10.1073/pnas.0932778100
发表时间：
2003-07-08
影响因子：
11.1
作者：
Lieberman, SM;Evans, AM;DiLorenzo, TP
通讯作者：
DiLorenzo, TP
影响因子：
6.4
作者：
Penaloza-MacMaster, Pablo;Masopust, David;Ahmed, Rafi
通讯作者：
Ahmed, Rafi
影响因子：
4.8
作者：
Jost, Lou
通讯作者：
Jost, Lou