MRNIP is essential for meiotic progression and spermatogenesis in mice
MRNIP is essential for meiotic progression and spermatogenesis in mice
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MRNIP 对于小鼠减数分裂进程和精子发生至关重要
DOI：
10.1016/j.bbrc.2021.02.143
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发表时间：
2021-03-06
影响因子：
3.1
通讯作者：
Huang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Lin, Meng;Lv, Jinxing;Huang, Xiaoyan
减数分裂同源重组(HR)始于DNA双链断裂(DSB)的程序性产生，导致遗传信息的交换和基因组的多样性。这一过程需要Mre11-Rad50-NBS1(MRN)复合体的紧密合作，以促进DSB的形成和DNA末端切除。然而，对MRN复合体的调控机制仍有待探索。在本研究中，我们报道了MRN相互作用蛋白，MRNIP，是一个新的HR因子，对MRN复合体的表达和重组酶DMC1/RAD51的装载至关重要。在小鼠中敲除mrnip基因导致突触异常、心率受损和男性不育症。综上所述，MRNIP是一个新的HR因子，可能通过MRN复合体促进减数分裂进程。？2021爱思唯尔公司。保留所有权利。
Meiotic homologous recombination (HR) initiates with the programmed generation of DNA double strand breaks (DSBs), which result in the exchange of genetic information and genome diversity. This process requires the tight cooperation of the MRE11-RAD50-NBS1 (MRN) complex to promote DSB formation and DNA end resection. However, the mechanism regulating MRN complex remains to be explored. In the present study, we report that MRN-interacting protein, MRNIP, is a novel factor for HR and is crucial for the expression of the MRN complex and loading of recombinases DMC1/RAD51. Knockout of Mrnip in mice led to aberrant synapsis, impaired HR, and male subfertility. In conclusion, MRNIP is a novel HR factor that probably promotes meiotic progression through the MRN complex. ? 2021 Elsevier Inc. All rights reserved.