Transcriptome Profiling Reveals Insight into Distinct Immune Responses to Aeromonas salmonicida in Gill of Two Rainbow Trout Strains
Transcriptome Profiling Reveals Insight into Distinct Immune Responses to Aeromonas salmonicida in Gill of Two Rainbow Trout Strains
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DOI：
10.1007/s10126-013-9552-x
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发表时间：
2014-06-01
影响因子：
3
通讯作者：
Goldammer, Tom
中科院分区：
文献类型：
--
作者：
Rebl, Alexander;Korytar, Tomas;Goldammer, Tom
鱼鳃是与水生环境沟通的重要器官。采用4x 44 K寡核苷酸微阵列技术，通过基因表达谱分析，检测了两个孵化场虹鳟鱼品系在注射强致病性杀鲑气单胞菌后鳃中的转录变化。重点放在“感染后第3天”，这是炎症消退的决定性时间点。比较鳃组织中差异调节的特征和途径，发现本地繁殖品系BORN和进口的美国虹鳟鱼采用共同和特异的免疫策略。在鳃感染BORN鳟鱼，我们观察到一个动态调节的基因控制NF-κ B B途径和诱导的因素，促进骨髓细胞的发展，而溶菌酶和免疫球蛋白基因的表达增加是明显的鳃感染进口鳟鱼。为了证明阵列预测的候选基因以及已知的免疫基因与鳃免疫的相关性，进行了随后的体外实验。总之，我们发现了动态的，但温和的变化，在广泛的免疫相关功能的表达，这意味着鳃的参与病原体防御策略。
The fish gills represent a crucial organ for the communication with the aquatic environment. Transcriptional changes in gills of two hatchery rainbow trout strains in response to injection with the potent pathogen Aeromonas salmonicida were detected by global gene expression profiling using a 4x44K oligonucleotide microarray. Emphasis was placed on "day 3 postinfection" representing a decisive time point for the resolution of inflammation. The comparison of features and pathways differentially regulated in branchial tissues revealed that the local breeding strain BORN and imported American rainbow trout apply common and specific immune strategies. In gills of infected BORN trout, we observed a dynamic regulation of genes controlling NF-kappa B pathways and the induction of factors promoting the development of myeloid cells, whereas an increased expression of lysozyme and immunoglobulin genes was obvious in gills of infected import trout. In order to prove the relevance of the array-predicted candidates as well as well-known immune genes for gill immunity, a subsequent in vitro experiment was conducted. Altogether, we uncovered dynamic but moderate changes in the expression of a broad range of immune-relevant features implying the gill's involvement in pathogen defense strategies.