Luteinizing hormone receptor, steroidogenesis acute regulatory protein, and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries.
Luteinizing hormone receptor, steroidogenesis acute regulatory protein, and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries.
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DOI：
10.1210/jcem.86.3.7318
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发表时间：
2001-03
期刊：
影响因子：
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通讯作者：
A. Jakimiuk;S. Weitsman;Alireza Navab;D. Magoffin
中科院分区：
文献类型：
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作者：
A. Jakimiuk;S. Weitsman;Alireza Navab;D. Magoffin
最近的研究表明，在多囊卵巢患者中，甾体生成酶信使核糖核酸（mrna）可能在鞘细胞中过度表达，而LH受体可能在颗粒细胞中过早表达。本研究的目的是确定多囊卵巢鞘和颗粒细胞中是否存在异常的基因表达。从12名多囊卵巢综合征妇女和24名定期月经的对照妇女中获得卵巢组织标本。从单个卵泡中显微解剖颗粒细胞和内膜。RT-PCR检测LH受体、甾体生成急性调节蛋白（StAR）、胆固醇侧链切割细胞色素P450 （CYP11A）和17 α -羟化酶/C（17-20）裂解酶细胞色素P450 (CYP17) mrna。3 ~ 7 mm对照卵泡和优势卵泡在鞘细胞和颗粒细胞中LH受体mRNA的表达没有差异。CYP11A和CYP17 mrna在3 ~ 7mm卵泡的鞘细胞中高于显性卵泡，但StAR的表达没有差异。在颗粒细胞中，优势卵泡中StAR和CYP11A mRNA的表达高于3 ~ 7mm卵泡。PCOS卵泡鞘细胞中LH受体、StAR、CYP11A和CYP17 mRNA的平均表达水平高于大小匹配的对照卵泡。在颗粒细胞中，PCOS的LH受体和CYP11A mRNA的平均表达水平高于对照卵泡，而StAR mRNA的平均表达水平不高于对照卵泡。这些数据表明，调控蛋白和甾体生成酶mrna在多囊卵巢的鞘细胞和颗粒细胞中过表达，支持鞘细胞过度刺激和颗粒细胞可能过早黄体生成的结论。
Recent data suggest that steroidogenic enzyme messenger ribonucleic acids (mRNAs) may be overexpressed in thecal cells, and LH receptors may be prematurely expressed in granulosa cells in women with polycystic ovaries. The purpose of this study was to determine whether there is abnormal gene expression in thecal and granulosa cells from polycystic ovaries. Ovarian tissue specimens were obtained from 12 women with PCOS and 24 regularly cycling control women. The granulosa cells and the theca interna were microdissected from individual follicles. LH receptor, steroidogenesis acute regulatory protein (StAR), cholesterol side-chain cleavage cytochrome P450 (CYP11A), and 17alpha-hydroxylase/C(17-20) lyase cytochrome P450 (CYP17) mRNAs were measured by RT-PCR. There was no difference between 3- to 7-mm control follicles and dominant follicles with respect to LH receptor mRNA expression in either thecal or granulosa cells. CYP11A and CYP17 mRNAs were higher in thecal cells from 3- to 7-mm follicles than in dominant follicles, but StAR expression was not different. In granulosa cells, StAR and CYP11A mRNA expression was higher in dominant follicles than in 3- to 7-mm follicles. The mean levels of LH receptor, StAR, CYP11A, and CYP17 mRNA expression were higher in thecal cells from PCOS follicles than in size-matched control follicles. In granulosa cells, the mean levels of LH receptor and CYP11A, but not StAR, mRNA expression were higher in PCOS than in control follicles. These data demonstrate that regulatory protein and steroidogenic enzyme mRNAs are overexpressed in thecal and granulosa cells from polycystic ovaries and support the conclusions that the thecal cells are hyperstimulated and the granulosa cells may be prematurely luteinizing.