IVF of mouse ova in a simplex optimized medium supplemented with amino acids
IVF of mouse ova in a simplex optimized medium supplemented with amino acids
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DOI：
10.1093/humrep/15.8.1791
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发表时间：
2000-08-01
影响因子：
6.1
通讯作者：
Biggers, JD
中科院分区：
文献类型：
--
作者：
Summers, MC;McGinnis, LK;Biggers, JD
在改良单纯优化培养基（mKSOM）中添加氨基酸并没有增加CF1小鼠体外受精卵发育成囊胚的百分比。相反，开始孵化的囊胚百分比和这些囊胚中的细胞数量，特别是内细胞团中的细胞数量增加。添加的氨基酸还支持在相同的囊胚中形成更有组织的细胞外基质。结果表明，在氨基酸补充的mKSOM中产生的受精卵具有更大的发育潜力，也许发展速度更快，比mKSOM中产生的受精卵，这种增强的发育潜力可能是由于减轻渗透压对卵子和受精卵的氨基酸是渗透剂。人卵细胞体外受精可能受益于在培养液中加入游离氨基酸。可用于支持小鼠IVF和植入前发育的培养基的可用性可能有利于从冷冻保存的精子中恢复小鼠品系。
The addition of amino acids to a modified simplex optimized medium (mKSOM) did not increase the percentage of blastocysts that develop from CF1 mouse ova fertilized in vitro. In contrast, the percentage of blastocysts that began to hatch and the number of cells in these blastocysts, particularly in the inner cell mass, was increased. The added amino acids also supported the development of a more organized extracellular matrix in the same blastocysts. The results suggest that zygotes produced in amino acid-supplemented mKSOM have a greater developmental potential, perhaps developing at a faster rate, than zygotes produced in mKSOM, This enhanced developmental potential may be caused by the alleviation of osmotic stress on the ova and zygotes by the amino acids that are osmolytes. The fertilization of human ova in vitro may benefit from the inclusion of free amino acids in the fertilizing medium. The availability of a medium that can be used to support both IVF and preimplantation development in the mouse is likely to benefit the recovery of mouse strains from cryopreserved spermatozoa.