Selective binding of the estradiol receptor to a region at least one kilobase upstream from the rat prolactin gene.
Selective binding of the estradiol receptor to a region at least one kilobase upstream from the rat prolactin gene.
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雌二醇受体与大鼠催乳素基因上游至少一千碱基的区域选择性结合。
DOI：
10.1089/dna.1985.4.1
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Maurer,RA
中科院分区：
文献类型：
--
作者：
Maurer,RA
使用竞争性结合测定研究了雌二醇受体与催乳素基因克隆 DNA 片段的相互作用。大鼠催乳素基因 5' 侧翼区域的 DNA 片段能够选择性地结合雌二醇受体。代表催乳素基因剩余 10 kb 大部分的 DNA 片段显示出很少或没有与雌二醇受体的选择性结合。选择性结合受体的 5' 侧翼区域片段位于转录起始位点上游 1.2 至 2.0 kb 之间，而不是像在其他几种类固醇激素受体与特定基因的相互作用中观察到的那样位于更近的位置。核苷酸序列分析表明该DNA区域包含两个大片段的交替嘌呤-嘧啶序列。嘌呤-嘧啶交替区域之一非常大，含有超过 160 个几乎完美的聚（dG-dT）核苷酸。需要进一步的研究来确定受体是否与这些有趣的序列特异性相互作用。然而，雌二醇受体与催乳素基因上游 5' 侧翼区域结合的能力可能是允许受体选择性刺激该基因转录的机制的一部分。
The interaction of the estradiol receptor with cloned DNA fragments from the prolactin gene was investigated using a competitive binding assay. A DNA fragment from the 5′-flanking region of the rat prolactin gene was able to bind the estradiol receptor selectively. DNA fragments representing most of the remaining 10 kb of the prolactin gene showed little or no selective binding to the estradiol receptor. The fragment from the 5′-flanking region which selectively binds the receptor is located between 1.2 and 2.0 kb upstream from the transcription initiation site rather than in a more proximal position as has been observed for the interaction of several other steroid hormone receptors with specific genes. Nucleotide sequence analysis demonstrated that this region of DNA contains two large segments of alternating purine-pyrimidine sequence. One of the alternating purine-pyrimidine regions is very large, containing more than 160 nucleotides of almost perfect poly(dG-dT). Further studies will be required to determine if the receptor interacts specifically with these interesting sequences. However, the ability of the estradiol receptor to bind to an upstream 5′-flanking region of the prolactin gene may be part of the mechanism which allows the receptor to stimulate the transcription of this gene selectively.