DIRECT MEASUREMENT OF THE TRANSPORT-PROPERTIES OF A SINGLE DNA MOLECULE
DIRECT MEASUREMENT OF THE TRANSPORT-PROPERTIES OF A SINGLE DNA MOLECULE
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DOI：
10.1103/physrevlett.75.2436
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发表时间：
1995-09-18
影响因子：
8.6
通讯作者：
WIRTZ, D
中科院分区：
文献类型：
--
作者：
WIRTZ, D
将荧光标记的DNA分子末端拴在一个小磁珠上，并通过校准的磁力牵引通过未标记DNA分子的溶液，以监测链的传输系数。从力-速度图F-V，我们报告的第一个直接测量的摩擦系数zeta = F/V的一个单一的DNA分子。zeta被发现是高度依赖于速度，由于急剧的构象转变，增加速度与最近的模型由Ajdari等人。
A fluorescently labeled DNA molecule is end tethered to a small magnetic bead and pulled by a calibrated magnetic force through a solution of unlabeled DNA molecules to monitor the transport coefficients of the chain. From the force velocity diagram F-V, we report the first direct measurement of the friction coefficient zeta = F/V of a single DNA molecule. zeta is found to be highly velocity dependent due to sharp conformational transitions for increasing velocities in agreement with a recent model by Ajdari et al.