Organosulfur volatile profiles in Italian red garlic (Allium Sativum L.) varieties investigated by HS-SPME/GC-MS and chemometrics
Organosulfur volatile profiles in Italian red garlic (Allium Sativum L.) varieties investigated by HS-SPME/GC-MS and chemometrics
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DOI：
10.1016/j.foodcont.2021.108477
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发表时间：
2021-08-27
期刊：
影响因子：
6
通讯作者：
D'Archivio, Angelo Antonio
中科院分区：
文献类型：
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作者：
Biancolillo, Alessandra;Aloia, Rosaria;D'Archivio, Angelo Antonio
采用顶空固相微萃取-气相色谱-质谱联用技术（HS-SPME-GC-MS）对意大利4个地区的68个红蒜样品进行了分析，以探讨红蒜的挥发性成分是否与产地有关。通过Box-Behnken实验设计优化了HS-SPME/GC-MS分析。一旦收集GC-MS信号并鉴定挥发物，就尝试通过偏最小二乘判别分析（PLS-DA）进行地理分类。为了进行模型的外部验证，将调查的样本分为42个样本的训练集和26个样本的测试集。PLS-DA模型对21个外部样本进行了正确分类。最终，可变重要性投影（VIP）分析确定了13种有机硫化合物（两种硫化物，八种二硫化物和三种三硫化物），其中25种检测到的化合物，显示大蒜风味的含量显着依赖于灯泡的地理来源。
Sixty-eight samples of red garlic harvested in four Italian areas were analyzed by means of head-space solid phase micro-extraction (HS-SPME) coupled to gas-chromatography with mass spectrometry (GC-MS) to test whether the volatile composition is related with the geographical origin of the bulbs. HS-SPME/GC-MS analysis was optimized by means of a Box-Behnken design of experiment. Once GC-MS signals were collected and the volatiles identified, geographical classification was attempted by Partial Least Squares Discriminant Analysis (PLS-DA). To perform the external validation of the model, the investigated samples were divided into a training and a test set of 42 and 26 samples, respectively. The PLS-DA model correctly classified 21 external samples. Eventually, Variable Importance in Projection (VIP) analysis identified 13 organosulfur compounds (two sulphides, eight disulphides and three trisulphides), among the 25 detected ones, displaying a content in garlic flavour significantly dependent on the geographical origin of the bulbs.