Targeted metabolomics study of serum bile acid profile in patients with end-stage renal disease undergoing hemodialysis
Targeted metabolomics study of serum bile acid profile in patients with end-stage renal disease undergoing hemodialysis
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DOI：
10.7717/peerj.7145
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发表时间：
2019-06-17
期刊：
影响因子：
2.7
通讯作者：
Zhong, Ling
中科院分区：
文献类型：
--
作者：
Li, Rong;Zeng, Li;Zhong, Ling
背景：胆汁酸是肠道菌群的重要代谢产物，对宿主的健康有着深远的影响。然而，胆汁酸代谢是否参与终末期肾病（ESRD）的代谢并发症，以及胆汁酸对ESRD预后的影响仍不清楚。因此，本研究探讨胆汁酸谱改变与预后的ESRD patients.Methods：基于超高效液相色谱-串联质谱（UPLC-MS/MS）的靶向代谢组学方法被用来确定血清胆汁酸的变化ESRD患者（n = 77）和健康对照组（n = 30）之间的关系。进行单因素和多因素统计分析，筛选两组之间的胆汁酸的差异比例。结果：6个差异表达的胆汁酸被确定为潜在的生物标志物，用于区分ESRD患者和健康受试者。鹅去氧胆酸、脱氧胆酸和胆酸浓度降低与ESRD患者血脂异常显著相关。亚组分析显示，ESRD患者血清中牛磺胆酸、牛磺鹅去氧胆酸、牛磺猪胆酸和牛磺α-鼠胆酸浓度的显著升高与ESRD患者的不良预后相关。此外，血清胆汁酸谱的改变可能导致ESRD患者的不良预后和代谢并发症。
Background: Bile acids are important metabolites of intestinal microbiota, which have profound effects on host health. However, whether metabolism of bile acids is involved in the metabolic complications of end-stage renal disease (ESRD), and the effects of bile acids on the prognosis of ESRD remain obscure. Therefore, this study investigated the relationship between altered bile acid profile and the prognosis of ESRD patients.Methods: A targeted metabolomics approach based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to determine the changes in serum bile acids between ESRD patients (n = 77) and healthy controls (n = 30). Univariate and multivariate statistical analyses were performed to screen the differential proportions of bile acids between the two groups.Results: Six differentially expressed bile acids were identified as potential biomarkers for differentiating ESRD patients from healthy subjects. The decreased concentrations of chenodeoxycholic acid, deoxycholic acid and cholic acid were significantly associated with dyslipidemia in ESRD patients. Subgroup analyses revealed that the significantly increased concentrations of taurocholic acid, taurochenodeoxycholic acid, taurohyocholic acid and tauro alpha-muricholic acid were correlated to the poor prognosis of ESRD patients.Conclusions: The serum bile acid profile of ESRD patients differed significantly from that of healthy controls. In addition, the altered serum bile acid profile might contribute to the poor prognosis and metabolic complications of ESRD patients.