Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
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果蝇TRF2和TAF9调节脂滴大小和磷脂脂肪酸组成
DOI：
10.1371/journal.pgen.1006664
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发表时间：
2017-03
期刊：
影响因子：
4.5
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
Fan W;Lam SM;Xin J;Yang X;Liu Z;Liu Y;Wang Y;Shui G;Huang X
通用转录因子 TBP（TATA-box 结合蛋白）及其相关因子 (TAF) 共同形成 TFIID 复合物，指导转录起始。通过 RNAi 和突变分析，我们鉴定了一种特定的 TBP 家族蛋白 TRF2 和一组调节果蝇幼虫脂肪体中脂滴 (LD) 大小的 TAF。在果蝇的三个 TBP 基因 trf2、tbp 和 trf1 中，只有 trf2 功能丧失才会导致 LD 大小增加。此外，TRF2 和 TAF9 调节几类磷脂的脂肪酸组成。通过RNA分析，我们发现TRF2和TAF9影响一组常见基因的转录，包括影响磷脂脂肪酸组成的过氧化物酶体脂肪酸β-氧化相关基因。我们还发现几个 TRF2 和 TAF9 靶基因的敲低会导致大的 LD，这种表型与 trf2 突变体相似。总之，这些发现为一般转录机制在脂质稳态中的具体作用提供了新的见解。
The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid β-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis.