DNA Hybridization-Mediated Liposome Fusion at the Aqueous Liquid Crystal Interface.
DNA Hybridization-Mediated Liposome Fusion at the Aqueous Liquid Crystal Interface.
复制标题
DNA杂交介导的脂质体融合在水性液晶界面处。
DOI：
10.1002/adfm.201303885
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发表时间：
2014-06-04
影响因子：
19
通讯作者：
Schwartz, Daniel K.
中科院分区：
文献类型：
--
作者：
Noonan, Patrick S.;Mohan, Praveena;Goodwin, Andrew P.;Schwartz, Daniel K.
受体介导的双分子层融合在细胞生物学中的重要性促使了研究融合机制的仿生策略的发展。本文报道了一种利用液晶（LC）独特的物理和光学性质监测受体介导的融合的方法。PEG官能化脂质用于产生能够抑制脂质体与水/LC界面自发融合的界面环境。然后，利用本体相脂质体内的寡核苷酸和PEG-脂质单层在水/LC界面处的DNA杂交来诱导受体介导的脂质体融合。这些杂交事件在脂质体双层内诱导应变，促进脂质与LC界面混合，并因此产生有利于LC重新取向为垂面（垂直）状态的界面环境。此外，利用适体的双功能性通过调节适当配体的可用性来调节DNA杂交介导的脂质体融合（即，凝血酶）。这里，用于监测受体的基于LC的方法（即，DNA杂交）介导的脂质体融合的证明，脂质体的性质，决定融合动力学进行了探讨，并提供了一个例子，这种方法可以如何用于生物传感方案。
The prominence of receptor-mediated bilayer fusion in cellular biology motivates development of biomimetic strategies for studying fusogenic mechanisms. An approach is reported here for monitoring receptor-mediated fusion that exploits the unique physical and optical properties of liquid crystals (LC). PEG-functionalized lipids are used to create an interfacial environment capable of inhibiting spontaneous liposome fusion with an aqueous/LC interface. Then, DNA hybridization between oligonucleotides within bulk phase liposomes and a PEG-lipid monolayer at an aqueous/LC interface is exploited to induce receptor-mediated liposome fusion. These hybridization events induce strain within the liposome bilayer, promote lipid mixing with the LC interface, and consequently create an interfacial environment favoring re-orientation of the LC to a homeotropic (perpendicular) state. Furthermore, the bi-functionality of aptamers is exploited to modulate DNA hybridization-mediated liposome fusion by regulating the availability of the appropriate ligand (i.e., thrombin). Here, a LC-based approach for monitoring receptor (i.e., DNA hybridization)-mediated liposome fusion is demonstrated, liposome properties that dictate fusion dynamics are explored, and an example of how this approach may be used in a biosensing scheme is provided.
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