Muscle tissue kinetics of oxytetracycline following intramuscular and oral administration at two dosages to giant freshwater shrimp (Macrobrachium rosenbergii)
Muscle tissue kinetics of oxytetracycline following intramuscular and oral administration at two dosages to giant freshwater shrimp (Macrobrachium rosenbergii)
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DOI：
10.1111/j.1365-2885.2008.00988.x
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发表时间：
2008-12-01
影响因子：
1.3
通讯作者：
Kumagai, S.
中科院分区：
文献类型：
--
作者：
Poapolathep, A.;Poapolathep, S.;Kumagai, S.
罗氏沼虾（Macrobrachium rosenbergii）是泰国的一种本地物种，用于商业目的出口或供应以满足泰国当地的需求。主要抗生素土霉素（OTC）的药代动力学信息有限，可用于淡水虾。本研究的目的是研究在肌肉注射（i.m）或口服（p.o）两种剂量（11和22 mg/kg体重（b.w））给药后罗氏m.b robergii肌肉组织动力学。采用配备荧光检测器的高效液相色谱法测定虾组织中OTC的浓度。肌组织浓度分别在给药96 h和120 h后低于检测限（LOD, 0.1 μ g/g）。单次给药11 mg/kg体重时，肌肉峰值浓度（C-max）分别为3.47和1.73 μ g/g；单次给药22 mg/kg体重时，肌肉峰值浓度（C-max）分别为6.03和2.51 μ g/g。建立了非室室模型来描述OTC在巨型淡水虾体内的药代动力学。单次给药11 mg/kg b.w时，OTC的终末半衰期分别为28.68 h和28.09 h；单次给药22 mg/kg b.w时，OTC的终末半衰期分别为29.95 h和27.03 h。给药后的相对生物利用度分别为82.32%和64.67%。根据药代动力学数据，在大型淡水对虾养殖中，以11 mg/kg b.w.的剂量给药和口服OTC是合适的。为避免OTC药物残留在虾肌中，至少需要7个半衰期（8天）才能将药物从罗氏沼虾肌中清除。
The giant river shrimp (Macrobrachium rosenbergii), a native species of Thailand, is either exported for commercial purposes or supplied to meet the local requirements in Thailand. Limited pharmacokinetic information of the major antibiotic, oxytetracycline (OTC), is available for this freshwater shrimp. The purpose of the present study was to investigate the muscle tissue kinetics of OTC in M. rosenbergii following either intramuscular (i.m.) or oral (p.o.) administration at two dosages of 11 and 22 mg/kg body weight (b.w.). The concentration of OTC in shrimp tissues was measured using high-performance liquid chromatography (HPLC) equipped with a fluorescence detector. Muscle tissue concentrations were below the detection limit (LOD, 0.1 mu g/g) after 96 and 120 h, following i.m. and p.o. administration, respectively. Peak muscle concentrations (C-max) were 3.47 and 1.73 mu g/g after i.m. and p.o. administration at a single dose of 11 mg/kg b.w. whereas they were 6.03 and 2.51 mu g/g at a single dose of 22 mg/kg b.w., respectively. A noncompartment model was developed to describe the pharmacokinetics of OTC in the giant freshwater shrimp. The terminal half-lives of OTC were 28.68 and 28.09 h after i.m. and p.o. administration at a single dose of 11 mg/kg b.w., but 29.95 and 27.03 h at a single dose of 22 mg/kg b.w., respectively. The relative bioavailability was 82.32 and 64.67% following i.m. and p.o. administration, respectively. Based on the pharmacokinetic data, i.m. and p.o. administration with OTC at a dose of 11 mg/kg b.w. would be appropriate for use in giant freshwater shrimp farming. To avoid the OTC residue in shrimp muscle, it should take at least seven half-lives (8 days) to wash out the drug from the muscle of M. rosenbergii.