ENDOTHELIAL NITRIC-OXIDE SYNTHASE LOCALIZED TO HIPPOCAMPAL PYRAMIDAL CELLS - IMPLICATIONS FOR SYNAPTIC PLASTICITY
ENDOTHELIAL NITRIC-OXIDE SYNTHASE LOCALIZED TO HIPPOCAMPAL PYRAMIDAL CELLS - IMPLICATIONS FOR SYNAPTIC PLASTICITY
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DOI：
10.1073/pnas.91.10.4214
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发表时间：
1994-05-10
影响因子：
11.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
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作者：
DINERMAN, JL;DAWSON, TM;SNYDER, SH
使用选择性地与内皮型一氧化氮合酶（eNOS）独特的肽序列反应的抗体，我们证明了在大脑中的神经元群体的定位。在某些脑区，如小脑和嗅球，eNOS和神经元NOS（nNOS）出现在相同的细胞群中，但比例不同。在海马中，这两种酶的定位是惊人的不同，eNOS更集中在海马锥体细胞比在任何其他脑区，而nNOS仅限于偶尔的中间神经元。在许多脑区，NADPH黄递酶染色反映NOS催化活性。海马锥体细胞不染色心肌黄酶与传统的多聚甲醛固定，但染色强烈与戊二醛固定剂，大概反映eNOS催化活性。海马锥体细胞中的eNOS可能产生NO，NO被认为是长时程增强的逆行信使。
Using antibodies that react selectively with peptide sequences unique to endothelial nitric oxide synthase (eNOS), we demonstrate localizations to neuronal populations in the brain. In some brain regions, such as the cerebellum and olfactory bulb, eNOS and neuronal NOS (nNOS) occur in the same cell populations, though in differing proportions. In the hippocampus, localizations of the two enzymes are strikingly different, with eNOS more concentrated in hippocampal pyramidal cells than in any other brain area, whereas nNOS is restricted to occasional interneurons. In many brain regions NADPH diaphorase staining reflects NOS catalytic activity. Hippocampal pyramidal cells do not stain for diaphorase with conventional paraformaldehyde fixation but stain robustly with glutaraldehyde fixatives, presumably reflecting eNOS catalytic activity. eNOS in hippocampal pyramidal cells may generate the NO that has been postulated as a retrograde messenger of long-term potentiation.