The biotransformation of allyl alcohol and acrolein in rat liver and lung preparations.
The biotransformation of allyl alcohol and acrolein in rat liver and lung preparations.
复制标题
烯丙醇和丙烯醛在大鼠肝和肺制剂中的生物转化。
DOI：
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发表时间：
1980
影响因子：
3.9
通讯作者：
K. Leibman
中科院分区：
文献类型：
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作者：
J. M. Patel;J. C. Wood;K. Leibman
通过测定丙烯醛、丙烯酸、缩水甘油和乙二醛，研究了丙烯醇和丙烯醛在大鼠肺和肝制剂中的生物转化。从含有烯丙醇、NAD+和肝脏9000g上清液或胞浆的孵育混合物中用高压液相色谱检测到丙烯醛。当肺部分以类似方式处理时，未形成丙烯醛。在孵化混合物中加入吡唑抑制了反应。证明了丙烯醛在肝脏9000g上清液、胞浆和微粒体中代谢为丙烯酸，在这三个组分中，NAD+比NADP+形成更多的丙烯酸。丙烯酸也是由烯丙醇生成的。双硫兰可抑制NAD+和NADP+依赖的反应。当使用肺制剂时，没有形成丙烯酸。肺和肝微粒体烯丙醇和丙烯醛的环氧化产物已被鉴定。肝环氧化物水合酶可将缩水甘油转化为甘油，将乙二醛转化为甘油醛。环氧化物、缩水甘油和乙二醛也被发现是肺和肝脏胞浆谷胱甘肽S转移酶的底物。
The biotransformation of allyl alcohol and acrolein by rat lung and liver preparations was investigated by measuring acrolein, acrylic acid, glycidol, and glycidaldehyde. Acrolein was detected by high-pressure liquid chromatography from incubation mixtures containing allyl alcohol, NAD+, and liver 9000g supernatant fraction or cytosol. Acrolein was not formed when lung fractions were treated similarly. Addition of pyrazole in the incubation mixture inhibited the reaction. The metabolism of acrolein to acrylic acid by liver 9000g supernatant fraction, cytosol, and microsomes has been demonstrated; acrylic acid formation was greater with NAD+ than with NADP+ in all three fractions. Acrylic acid was also formed from allyl alcohol. Disulfiram inhibited the NAD+- and NADP+-dependent reactions. Acrylic acid was not formed when lung preparations were used. Lung and liver microsomal epoxidation products of allyl alcohol and acrolein have been identified. Conversion of glycidol to glycerol and glycidaldehyde to glyceraldehyde by liver epoxide hydrase has been demonstrated. Epoxides, glycidol, and glycidaldehyde were also found to be substrates for lung and liver cytosolic glutathione S-transferase.