Molecular detection of kobuviruses and recombinant noroviruses in cattle in continental Europe
Molecular detection of kobuviruses and recombinant noroviruses in cattle in continental Europe
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DOI：
10.1007/s00705-009-0518-2
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发表时间：
2009-11-01
影响因子：
2.7
通讯作者：
Thiry, Etienne
中科院分区：
文献类型：
--
作者：
Mauroy, Axel;Scipioni, Alexandra;Thiry, Etienne
牛诺如病毒有两种基因型（Jena和Newbury2）和两种基因型间重组株。几项研究表明，牛感染纽伯里2相关（基因型2）菌株的明显优势。牛粪便样品用靶向聚合酶和衣壳基因的两个引物对筛选。在占优势的基因型2序列中，两个与重组菌株Thirsk10遗传相关。检测到与Thirsk10遗传相关的序列，以及极低的Jena相关序列检测率，是比利时（欧洲大陆的代表性地区）牛诺如病毒种群的特征。出乎意料的是，牛科布病毒相关序列也被扩增，扩大了其在欧洲的分布区域。
Two genotypes (Jena and Newbury2) and two intergenotype recombinant strains have been recognized in bovine noroviruses. Several studies have shown an apparent predominance of bovine infection with Newbury2-related (genotype 2) strains. Bovine stool samples were screened with two primer pairs targeting both the polymerase and the capsid genes. Among the predominant genotype 2 sequences, two were genetically related to the recombinant strain Thirsk10. The detection of sequences genetically related to Thirsk10, together with the very low rate of detection of Jena-related sequences, characterized the bovine norovirus population in Belgium, a representative region of continental Europe. Unexpectedly, bovine kobuvirus-related sequences were also amplified, extending their distribution area in Europe.