Single-molecule fluorescence resonance energy transfer
Single-molecule fluorescence resonance energy transfer
复制标题
DOI：
10.1006/meth.2001.1217
复制
发表时间：
2001-09-01
期刊：
影响因子：
4.8
通讯作者：
Ha, T
中科院分区：
文献类型：
--
作者：
Ha, T
荧光共振能量转移(FRET)是研究生物分子构象分布和动力学的有力工具。一些构象变化很难同步，或者太罕见，无法使用系综FRET检测到。在单分子水平上检测到的FRET为探索结构变化的详细动力学开辟了新的机会，而不需要同步。在这里，我们讨论了实施它的实际考虑因素，包括实验仪器、荧光探针的选择、表面固定、单分子FRET分析方案和解释。(C)2001年学术出版社。
Fluorescent resonance energy transfer (FRET) is a powerful technique for studying conformational distribution and dynamics of biological molecules. Some conformational changes are difficult to synchronize or too rare to detect using ensemble FRET. FRET, detected at the single-molecule level, opens up new opportunities to probe the detailed kinetics of structural changes without the need for synchronization. Here, we discuss practical considerations for its implementation including experimental apparatus, fluorescent probe selection, surface Immobilization, single-molecule FRET analysis schemes, and interpretation. (C) 2001 Academic Press.