USE OF AN AMINO-CUPRIC-SILVER TECHNIQUE FOR THE DETECTION OF EARLY AND SEMIACUTE NEURONAL DEGENERATION CAUSED BY NEUROTOXICANTS, HYPOXIA, AND PHYSICAL TRAUMA
USE OF AN AMINO-CUPRIC-SILVER TECHNIQUE FOR THE DETECTION OF EARLY AND SEMIACUTE NEURONAL DEGENERATION CAUSED BY NEUROTOXICANTS, HYPOXIA, AND PHYSICAL TRAUMA
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DOI：
10.1016/0892-0362(94)90033-7
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发表时间：
1994-11-01
影响因子：
2.9
通讯作者：
DELORENZO, SDO
中科院分区：
文献类型：
--
作者：
DEOLMOS, JS;BELTRAMINO, CA;DELORENZO, SDO
本文介绍了一种新的检测神经元变性的氨基铜银方案。我们描述了它在大鼠脑内或全身注射各种神经毒物以及沙土鼠短暂性脑缺血发作后对早期和半急性变性进行可视化的选择性。早在物理创伤后5分钟，或纹状体内注射谷氨酸类似物或暴露于缺血发作后15分钟，在创伤的主要部位就有明显的神经元银染(I.G.注射部位)和与血液学有关的次级部位。外周注射三甲基锡(IP)中毒或脑内注射阿霉素中毒，分别在24小时或9-13天后可见可复制的变性模式。氨基铜银方法可以在清晰的背景下同时检测所有神经元的隔间。在进行性病理过程中，可以准确地追踪神经元胞体、树突、轴突和终末的退化以及新结构的招募。新试剂的加入提高了与以前版本的铜银法相比的灵敏度。文中详细讨论了该方法与其他神经毒性评价方法的优缺点，特别强调了该方法在区分不可逆变性和轴突变性方面的独特能力。在细胞体看起来完好无损的情况下。氨基铜银方法是在基础神经科学研究以及神经病理学和神经毒性评估中测量神经元损伤的一种特别有用的工具。
A new amino-cupric silver protocol is described for detection of neuronal degeneration. We describe its selectivity in visualizing both early and semiacute degeneration after intracerebral or systemic administration of a variety of neurotoxicants in rats, and after transient ischemic episodes in gerbils. As early as 5 min after physical trauma, or 15 min following either intrastriatal injections of glutamate analogs or exposure to ischemic episodes, neuronal silver staining was evident at primary sites of trauma (i.g. injection sites) and at hodologically related secondary sites. With intoxication by peripheral injections of trimethyltin (IP) or intracerebral injections of Doxorubicin, reproducible patterns of degeneration are demonstrable after 24 h or after 9-13 days, respectively. The amino-cupric silver method permits simultaneous detection of all neuronal compartments against a clear background. Degeneration in the neuronal cell bodies, dendrites, axons and terminals, as well as the recruitment of new structures in a progressive pathologic process, could be accurately followed. The inclusion of new reagents increased the sensitivity vis-a-vis previous versions of the cupric-silver method. The advantages and disadvantages of the current method in comparison with other means of neurotoxic assessment are discussed in detail, with special emphasis on its unique ability to discriminate irreversible degenerative phenomena and degeneration of axonal. components in cases where the cell body remains apparently intact. The amino-cupric silver method is an especially useful tool for surveying neuronal damage in basic neuroscience investigations and in neuropathologic and neurotoxic assessment.