Individual and combined effects of the infrared, visible, and ultraviolet light components of solar radiation on damage biomarkers in human skin cells
Individual and combined effects of the infrared, visible, and ultraviolet light components of solar radiation on damage biomarkers in human skin cells
复制标题
DOI：
10.1096/fj.201902351rr
复制
发表时间：
2020-01-16
期刊：
影响因子：
4.8
通讯作者：
Birch-Machin, Mark A.
中科院分区：
文献类型：
--
作者：
Hudson, Laura;Rashdan, Eyman;Birch-Machin, Mark A.
太阳紫外线（UV）诱发皮肤癌和光老化的能力是公认的。太阳辐射的红外（IR）和可见光（Vis）成分对皮肤的影响及其与紫外线的相互作用尚不为人所知。本研究比较了生理相关剂量的完全（UV + Vis + IR）太阳模拟光及其单个成分对来自人类供体的匹配的初级真皮成纤维细胞和表皮角质形成细胞对细胞损伤的三种生物标志物（活性氧（ROS）产生、线粒体DNA （mtDNA）和核DNA （nDNA）损伤）的影响。与原代角质形成细胞相比，太阳模拟光的可见光和红外成分在原代成纤维细胞中诱导的ROS、mtDNA和nDNA损伤更大（P < 0.001）。单独或联合暴露于特定太阳光线成分的实验表明，太阳光线的UV、Vis和IR成分协同增加了原代成纤维细胞的ROS生成，而不是原代角化细胞（P < .001）。皮肤细胞系被用来证实这些发现。这些观察结果对不同类型的皮肤细胞对太阳光线的个体和相互作用成分的反应以及因此的光损伤机制和光保护干预具有重要意义。
The ability of solar ultraviolet (UV) to induce skin cancer and photoaging is well recognized. The effect of the infrared (IR) and visible light (Vis) components of solar radiation on skin and their interaction with UV is less well known. This study compared the effects of physiologically relevant doses of complete (UV + Vis + IR) solar-simulated light and its individual components on matched primary dermal fibroblasts and epidermal keratinocytes from human donors on three biomarkers of cellular damage (reactive oxygen species (ROS) generation, mitochondrial DNA (mtDNA), and nuclear DNA (nDNA) damage). There was a greater induction of ROS, mtDNA, and nDNA damage with the inclusion of the visible and IR components of solar-simulated light in primary fibroblast cells compared to primary keratinocytes (P < .001). Experiments using exposure to specific components of solar light alone or in combination showed that the UV, Vis, and IR components of solar light synergistically increased ROS generation in primary fibroblasts but not primary keratinocytes (P < .001). Skin cell lines were used to confirm these findings. These observations have important implications for different skin cell type responses to the individual and interacting components of solar light and therefore photodamage mechanisms and photoprotection interventions.