Organization of the 3'-terminal half of beet yellow stunt virus genome and implications for the evolution of closteroviruses
Organization of the 3'-terminal half of beet yellow stunt virus genome and implications for the evolution of closteroviruses
复制标题
DOI：
10.1006/viro.1996.0366
复制
发表时间：
1996-07-01
期刊：
影响因子：
3.7
通讯作者：
Dawson, WO
中科院分区：
文献类型：
--
作者：
Karasev, AV;Nikolaeva, OV;Dawson, WO
甜菜黄矮化病毒 (BYSV) 基因组 3' 末端的一半（10,545 nt）已被克隆和测序。 BYSV 基因组的测序部分包含 10 个开放阅读框 (ORF) 和 241 nt 的 3' 非翻译区。该序列在5'至3'方向上跨越复制相关多蛋白基因(ORF la)的C端区域，其包括解旋酶(HEL)的典型基序组、整个53-kDa聚合酶(RdRp)基因(ORF Ib)和编码30-kDa(ORF 2)、6-kDa(ORF 3)、66-kDa(ORF 4)的基因， 61-kDa (ORF 5)、25-kDa (ORF 6)、23.7-kDa（外壳蛋白，CP）(ORF 7)、18-kDa (ORF 8) 和 22-kDa (ORF 9) 蛋白质。 BYSV 的双链 RNA“复制形式”被证明在 (+) 链的 3' 末端具有非模板 G 残基。 BYSV 的 RdRp 可能是通过 +1 核糖体移码表达的。在 BYSV 中鉴定出长线病毒中保守的五基因模块；它包括编码 6-kDa 小疏水蛋白、细胞 HSP70 热休克蛋白的 66-kDa 同源物、61-kDa 蛋白和 CP 的 25-kDa 分歧副本以及 CP 基因本身的基因阵列。对复制相关的 HEL 和 RdRp 结构域以及来自五基因模块的蛋白质的系统发育分析表明，BYSV 与其他两种线形病毒、甜菜黄病毒 (BYV) 和柑橘 tristeza (CTV) 病毒之间关系最密切。与 CTV 一样，BYSV 基因组在 RdRp 和 6 kDa 蛋白质基因之间包含一个 30 kDa 蛋白质基因，并且与 BW 一样，它在 CP 基因下游只有两个基因。 BYSV 基因组的组织似乎介于 BW 和 CN 之间，这表明这三种病毒可能代表线形病毒进化中的三个不同但可能接近的阶段。 (C) 1996 学术出版社
The 3'-terminal half of the beet yellow stunt virus (BYSV) genome, 10,545 nt, has been cloned and sequenced. The sequenced portion of the BYSV genome encompasses 10 open reading frames (ORFs) and 241 nt of the 3' untranslated region. The sequence spans, in the 5' to 3' direction, the C-terminal region of the replication-associated polyprotein gene (ORF la) which includes the set of motifs typical of helicases (HEL), the entire 53-kDa polymerase (RdRp) gene (ORF Ib), and genes encoding 30-kDa (ORF 2), 6-kDa (ORF 3), 66-kDa (ORF 4), 61-kDa (ORF 5), 25-kDa (ORF 6), 23.7-kDa (coat protein, CP) (ORF 7), 18-kDa (ORF 8), and 22-kDa (ORF 9) proteins. The double-stranded RNA ''replicative form'' of the BYSV was demonstrated to have a nontemplate G residue at the 3' terminus of the (+) strand. The RdRp of BYSV is presumably expressed via a +1 ribosomal frameshift. The five-gene module conserved among closteroviruses was identified in BYSV; it includes a gene array coding for a 6-kDa small hydrophobic protein, a 66-kDa homolog of the cellular HSP70 heat shock proteins, a 61-kDa protein, and a 25-kDa diverged copy of the CP followed by the CP gene itself. Phylogenetic analysis of the replication-associated HEL and RdRp domains as well as proteins from the five-gene module demonstrated the closest relationship between BYSV and two other closteroviruses, beet yellows (BYV) and citrus tristeza (CTV) viruses. Like CTV, the BYSV genome contains a 30-kDa protein gene between the RdRp and the 6-kDa protein genes, and like BW it has only two genes downstream of the CP gene. The organization of the BYSV genome appears to be intermediate between BW and CN, which suggests that these three viruses might represent three distinct but probably close stages in the closterovirus evolution. (C) 1996 Academic Press, Inc.