Effect of dietary daidzein on egg production, shell quality, and gene expression of ER-α, GH-R, and IGF-IR in shell glands of laying hens
Effect of dietary daidzein on egg production, shell quality, and gene expression of ER-α, GH-R, and IGF-IR in shell glands of laying hens
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DOI：
10.1021/jf071085r
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发表时间：
2007-08-22
影响因子：
6.1
通讯作者：
Zhao, Ruqian
中科院分区：
文献类型：
--
作者：
Ni, Yingdong;Zhu, Qian;Zhao, Ruqian
我们之前的研究表明，日粮中的大豆黄酮可以提高鸭子在产蛋周期后期的产蛋量。本研究旨在探讨大豆黄酮对蛋鸡的影响，重点关注蛋壳质量。测定蛋鸡壳腺中 ER-α、GH-R 和 IGF-IR mRNA 的表达，以确定黄豆苷元作用的靶基因，并揭示蛋鸡壳质量与蛋鸡壳腺基因表达谱之间的关系。将 1000 只 445 日龄的 ISA 母鸡随机分配到两组，并给予基础日粮，每公斤日粮添加或不添加 10 毫克黄豆苷元，持续 9 周。补充大豆苷元可显着提高产蛋率和饲料转化率。经大豆苷元处理的母鸡，蛋壳厚度增加，而破裂蛋的百分比降低。与对照组相比，大豆苷元治疗组的血清 E-2 和磷酸盐浓度没有改变，但血清 Ca2+ 水平和胫骨骨矿物质密度显着增加。在黄豆苷元处理的母鸡中，与输卵管重量显着增加同时，壳腺中 GH-R 和 IGF-IR mRNA 显着下调，并且 ER α mRNA 表达呈下降趋势。结果表明，日粮中的黄豆苷元可改善母鸡产蛋后期（高峰后）的产蛋性能和蛋壳质量，这与壳腺基因表达的调节有关。
Our previous studies demonstrated that dietary daidzein improves egg production in ducks during the late period of the laying cycle. The present study was aimed to investigate the effect of daidzein in laying hens, with more focus on eggshell quality. The expression of ER-alpha, GH-R, and IGF-IR mRNA in shell glands was determined to identify the target genes of daidzein action and to reveal the relationship between shell quality and profiles of gene expression in shell glands of laying hens. 1000 ISA hens, at 445 days of age, were allotted at random to two groups and given the basal diet with or without 10 mg of daidzein per kg diet for 9 weeks. Daidzein supplement significantly increased the egg laying rate and the feed conversion ratio. The eggshell thickness increased, while the percentage of cracked eggs decreased in daidzein-treated hens. Serum E-2 and phosphate concentrations were not altered, but the level of serum Ca2+ and the tibia bone mineral density were significantly increased in the daidzein-treated group compared with their control counterparts. In parallel with the significant increase of oviduct weight, significant down-regulation of GH-R and IGF-IR mRNA and a trend of decrease in ER alpha mRNA expression in shell glands were observed in daidzein-treated hens. The results indicate that dietary daidzein improves egg laying performance and eggshell quality during the late (postpeak) laying stage of hens, which is associated with modulations in gene expression in the shell gland.