DETECTION OF METABOLITES IN RABBIT BRAIN BY C-13 NMR-SPECTROSCOPY FOLLOWING ADMINISTRATION OF [1-C-13]GLUCOSE
DETECTION OF METABOLITES IN RABBIT BRAIN BY C-13 NMR-SPECTROSCOPY FOLLOWING ADMINISTRATION OF [1-C-13]GLUCOSE
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DOI：
10.1002/mrm.1910030611
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发表时间：
1986-12-01
影响因子：
3.3
通讯作者：
SHULMAN, RG
中科院分区：
文献类型：
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作者：
BEHAR, KL;PETROFF, OAC;SHULMAN, RG
静脉输注[1-13C]葡萄糖后，用表面线圈收集活体兔脑的 1H 解耦 13C NMR 谱 (20.2 MHz)。输注后 15 分钟内，.alpha。和.beta。检测到葡萄糖的端基异构体，不久之后，检测到谷氨酸和（或）谷氨酰胺的 C4、C3 和 C2 位碳原子。吸入氧从 30% 减少到 5% 后，检测到乳酸 C3。乳酸共振的强度在缺氧期间逐渐上升，然后在氧气恢复期间下降。通过 1H NMR 测量动脉血的 13C 同位素富集分数，提供有关血糖标记的速率和程度的信息。
1H-decoupled 13C NMR spectra (20.2 MHz) of the living rabbit brain were collected with a surface coil following the intravenous infsusion of [1-13C]glucose. Within 15 min of infusion, the .alpha. and .beta. anomers of glucose were detected and, shortly thereafter, the carbon atoms at positions C4, C3, and C2 of glutamate and(or) glutamine. After reductions of inspired oxygen from 30 to 5%, lactate C3 was detected. The intensity of the lactate resonance rose progressively during hypoxia and later fell during recovery with oxygen. The 13C fractional isotopic enrichment of arterial blood was measured by 1H NMR providing information on the rate and extent of blood glucose labeling.