Four receptor-like cytoplasmic kinases regulate development and immunity in rice.
Four receptor-like cytoplasmic kinases regulate development and immunity in rice.
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四种受体样细胞质激酶调节水稻的发育和免疫。
DOI：
10.1111/pce.12696
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Chen Xuewei
中科院分区：
文献类型：
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作者：
Zhou Xiaogang;Wang Jing;Peng Chunfang;Zhu Xiaobo;Yin Junjie;Li Weitao;He Min;Wang Jichun;Chern Mawsheng;Yuan Can;Wu Wenguan;Ma Weiwei;Qin Peng;Ma Bintian;Wu Xianjun;Li Shigui;Ronald Pamela;Chen Xuewei
受体样细胞质激酶（Receptor-like cytoplasmic kinases，RLCK）是植物中一类重要的蛋白质。然而，很少有RLCK已被很好地表征。在这里，我们报告了来自亚家族VII的四个水稻RLCK-OsRLCK 57、OsRLCK 107、OsRLCK 118和OsRLCK 176的功能特征。这些OsRLCK与水稻油菜素类固醇受体OsBRI 1相互作用，但不与XA 21免疫受体相互作用。与野生型水稻相比，这些基因中的每一个沉默的转基因株系具有扩大的叶角，并且对油菜素处理高度敏感。OsRLCK 57沉默的转基因植物具有显著更少的分蘖和减少的穗二次分枝，并且OsRLCK 107和OsRLCK 118沉默的品系产生更少的种子。这些基因的沉默降低了Xa 21基因的表达，并损害了Xa 21介导的对黄单胞菌致病型的免疫力。米。我们的研究表明，这些OsRLCK负调节BR信号传导，同时通过促进免疫受体XA 21的表达来正调节免疫反应。
Receptor-like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. However, few RLCKs have been well characterized. Here, we report the functional characterization of four rice RLCKs - OsRLCK57, OsRLCK107, OsRLCK118 and OsRLCK176 from subfamily VII. These OsRLCKs interact with the rice brassinosteroid receptor, OsBRI1 in yeast cell, but not the XA21 immune receptor. Transgenic lines silenced for each of these genes have enlarged leaf angles and are hypersensitive to brassinolide treatment compared to wild type rice. Transgenic plants silenced for OsRLCK57 had significantly fewer tillers and reduced panicle secondary branching, and lines silenced for OsRLCK107 and OsRLCK118 produce fewer seeds. Silencing of these genes decreased Xa21 gene expression and compromised XA21-mediated immunity to Xanthomonas oryzae pv. oryzae. Our study demonstrates that these OsRLCKs negatively regulate BR signalling, while positively regulating immune responses by contributing to the expression of the immune receptor XA21.