Dynamic equilibrium between cancer stem cells and non-stem cancer cells in human SW620 and MCF-7 cancer cell populations.
Dynamic equilibrium between cancer stem cells and non-stem cancer cells in human SW620 and MCF-7 cancer cell populations.
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人类SW620和MCF-7癌细胞群中癌症干细胞和非干癌细胞之间的动态平衡
DOI：
10.1038/bjc.2012.126
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发表时间：
2012-04-24
影响因子：
8.8
通讯作者：
Wang, Y.
中科院分区：
文献类型：
--
作者：
Yang, G.;Quan, Y.;Wang, W.;Fu, Q.;Wu, J.;Mei, T.;Li, J.;Tang, Y.;Luo, C.;Ouyang, Q.;Chen, S.;Wu, L.;Hei, T. K.;Wang, Y.
背景：肿瘤干细胞(CSCs)的研究范式表明，CSCs在癌症治疗中可能具有重要的临床意义。此前，我们曾报道CSCs在低LET和高LET照射后48h的聚集效率不同。方法：通过抗原图谱和球体形成等多种方法对肿瘤干细胞和非干细胞癌细胞(NSCCs)进行分选和功能鉴定。原位观察CSCs与NSCCs之间的相互转化。在正常和辐射环境下检测多代肿瘤干细胞比例。结果：CSCs和NSCs的表面抗原标志物存在内在的动态平衡状态，存在自发的和原位可见的相互转化，与既往的照射无关。加入转化生长因子-β-1可加速细胞间的平衡，抑制转化生长因子-β信号转导可扰乱细胞间的平衡，使细胞比例显著降低。结论：正常和辐射环境下CSCs与NSCCs之间存在着内在的相互转化和动态平衡，转化生长因子-β可能通过激活上皮间充质转化而在平衡中发挥重要作用。
Background:Cancer stem cells (CSCs) paradigm suggests that CSCs might have important clinical implications in cancer therapy. Previously, we reported that accumulation efficiency of CSCs is different post low-and high-LET irradiation in 48 h.Methods:Cancer stem cells and non-stem cancer cells (NSCCs) were sorted and functionally identified through a variety of assays such as antigen profiles and sphere formation. Inter-conversion between CSCs and NSCCs were in situ visualised. Cancer stem cells proportions were assayed over multiple generations under normal and irradiation surroundings. Supplement and inhibition of TGF-β1, as well as immunofluorescence assay of E-cadherin and Vimentin, were performed.Results:Surface antigen markers of CSCs and NSCCs exist in an intrinsic homoeostasis state with spontaneous and in situ visualisable inter-conversions, irrespective of prior radiations. Supplement with TGF-β1 accelerates the equilibrium, whereas inhibition of TGF-β signalling disturbs the equilibrium and significantly decreases CSC proportion. Epithelial mesenchymal transition (EMT) might be activated during the process.Conclusion:Our results indicate that the intrinsic inter-conversion and dynamic equilibrium between CSCs and NSCCs exist under normal and irradiation surroundings, and TGF-β might have important roles in the equilibrium through activating EMT.
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DOI：
10.1126/science.1171837
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rosen JM;Jordan CT
通讯作者：
Jordan CT
影响因子：
64.8
作者：
O'Brien, Catherine A.;Pollett, Aaron;Dick, John E.
通讯作者：
Dick, John E.
影响因子：
11.2
作者：
Cui, Xing;Oonishi, Kazuhiko;Okayasu, Ryuichi
通讯作者：
Okayasu, Ryuichi
DOI：
10.1074/jbc.m110.200675
发表时间：
2011-10-28
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Achuthan S;Santhoshkumar TR;Prabhakar J;Nair SA;Pillai MR
通讯作者：
Pillai MR
影响因子：
8
作者：
Kumar, S. M.;Liu, S.;Lu, H.;Zhang, H.;Zhang, P. J.;Gimotty, P. A.;Guerra, M.;Guo, W.;Xu, X.
通讯作者：
Xu, X.