The dog genome: Survey sequencing and comparative analysis
The dog genome: Survey sequencing and comparative analysis
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DOI：
10.1126/science.1086432
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发表时间：
2003-09-26
期刊：
影响因子：
56.9
通讯作者：
Venter, JC
中科院分区：
文献类型：
--
作者：
Kirkness, EF;Bafna, V;Venter, JC
对狗基因组序列的调查（622万序列读数，1.5倍覆盖率）证明了样本测序在哺乳动物基因组比较分析和产生物种特异性资源方面的力量。超过6.5亿个碱基对（bbb25%）的狗序列与人类基因组唯一一致，包括24,567个注释的人类基因中的18,473个假定的同源物片段。突变率、保守性、重复序列和系统发育可以在人类、小鼠和狗之间进行比较。多种多态性元素被确定，这将是有价值的绘制疾病和性状在狗的遗传基础。
A survey of the dog genome sequence (6.22 million sequence reads; 1.5X coverage) demonstrates the power of sample sequencing for comparative analysis of mammalian genomes and the generation of species-specific resources. More than 650 million base pairs (>25%) of dog sequence align uniquely to the human genome, including fragments of putative orthologs for 18,473 of 24,567 annotated human genes. Mutation rates, conserved synteny, repeat content, and phylogeny can be compared among human, mouse, and dog. A variety of polymorphic elements are identified that will be valuable for mapping the genetic basis of diseases and traits in the dog.