Use of TD-GC-TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa).
Use of TD-GC-TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa).
复制标题
DOI：
10.1016/j.foodchem.2015.08.043
复制
发表时间：
2016-03-01
期刊：
影响因子：
8.8
通讯作者：
Rogers HJ
中科院分区：
文献类型：
--
作者：
Bell L;Spadafora ND;Müller CT;Wagstaff C;Rogers HJ
我们提出了一种从火箭沙拉包装顶空进行 VOC 分析的可靠方法。 TD-GC-TOF-MS 假定鉴定出 39 种挥发性化合物。芥菜的 VOC 特征在不同种质之间存在显着差异。异硫氰酸酯化合物在保质期内会显着降解。 VOC 分布的变化可以为评估叶子质量提供有用的工具。营养强化品种育种的一个重要步骤是确定收获后供应链对植物化学物质的影响以及随时间推移产生的挥发性有机化合物的变化。使用 TD-GC-TOF-MS 并描述了使用便携式管从顶部空间提取 VOC 的技术。检测到 42 种化合物；通过与 NIST 文库比较，鉴定出 39 个。芥菜中有 35 种化合物以前从未报道过。对 7 份样品在 7 天内顶空 VOC 的变化进行了评估。 3 个时间点的 VOC 相对量存在显着差异——“第 0 天”含有异硫氰酸酯的分子含量丰富。每个品种显示出每天产生的挥发物的比例/类型的差异。 PCA 显示了根据采样日期的 VOC 分布情况。 VOC 分布随时间的变化可以提供评估保质期的工具。
We present a robust method for VOC analysis from rocket salad packaging headspace. TD-GC–TOF-MS putatively identified 39 volatile compounds. VOC profiles for Eruca sativa varied significantly between accessions. Isothiocyanate compounds degrade significantly over shelf-life. Changes in VOC profiles could provide a useful tool for assessment of leaf quality. An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD-GC–TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7 days. Relative amounts of VOCs across 3 time points were significantly different – isothiocyanate-containing molecules being abundant on ‘Day 0’. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf life.