Correlative association between circadian expression of mousePer2 gene and the proliferation of the neural stem cells
Correlative association between circadian expression of mousePer2 gene and the proliferation of the neural stem cells
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DOI：
10.1016/j.neuroscience.2007.02.018
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发表时间：
2007-05-11
期刊：
影响因子：
3.3
通讯作者：
Shinohara, K.
中科院分区：
文献类型：
--
作者：
Moriya, T.;Hiraishi, K.;Shinohara, K.
我们研究了小鼠周期（mPer）基因（mPer 1和mPer 2）的昼夜表达和体外神经干细胞的增殖。用神经球法从胚胎小鼠神经节隆起中扩增神经干细胞，然后用表皮生长因子（EGF）刺激其有丝分裂活性。用WST-8法和BrdU掺入法检测细胞增殖的时间进程，用RT-PCR和免疫细胞化学法检测mPer 1和mPer 2基因的表达。我们已经发现，EGF治疗引起的昼夜变化，在两个活细胞数和DNA合成活性的神经干细胞的增加。此外，mPer 2的基因表达，而不是mPer 1，有节奏地变化的一段时间为24小时，与DNA合成活性的节奏呈负相关。此外，用针对mPer 2的反义寡核苷酸处理增加了神经干细胞的DNA合成活性。这些结果表明，mPer 2可能周期性地抑制神经干细胞的增殖。(C)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
We examined the circadian expression of mouse-Period (mPer) genes (mPer1 and mPer2) and the proliferation of the neural stem cells in vitro. The neural stem cells from the ganglionic eminence of embryonic mice were expanded by the neurosphere method and then treated with epidermal growth factor (EGF) to stimulate their mitotic activity. The time courses of the proliferation were examined by WST-8 assay and bromodeoxyuridine (BrdU) incorporation assay and the expression of mPer1 and mPer2 genes was examined by RT-PCR and immunocytochemistry. We have found that EGF treatment elicited the circadian change in both the increase in viable cell number and DNA synthesis activity of the neural stem cells. Also, the gene expression of mPer2, but not mPer1, changed rhythmically with a period of 24 h and correlated negatively with the DNA synthesis activity rhythm. Furthermore, the treatment with an antisense oligonucleotide against mPer2 increased the DNA synthesis activity of the neural stem cells. These results suggest that mPer2 might periodically suppress the proliferation of neural stem cells. (C) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.