Erythrocytes fail to induce glutathione in response to diethyl maleate or hyperoxia.
Erythrocytes fail to induce glutathione in response to diethyl maleate or hyperoxia.
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红细胞无法响应马来酸二乙酯或高氧而诱导谷胱甘肽。
DOI：
10.1152/ajplung.1989.257.4.l272
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Fanburg,BL
中科院分区：
文献类型：
--
作者：
Phelps,DT;Deneke,SM;Baxter,DF;Fanburg,BL
我们先前发现，肺动脉内皮细胞暴露于高氧或低浓度的马来酸二乙酯(DEM)会导致细胞内谷胱甘肽(GSH)升高以及谷氨酸和半胱氨酸的摄取。目前的研究证实，这种升高发生在各种肺细胞(牛肺动脉内皮细胞和平滑肌细胞以及大鼠肺成纤维细胞和上皮样细胞)上，而不是人、大鼠和鸡的红细胞。事实上，当DEM浓度从0.05 mM降至0.5 mM时，人和大鼠红细胞的GSH水平显著降低，而0.05 mM的DEM几乎完全消除了鸡红细胞的GSH水平。此外，所有红细胞都未能积聚可测量的放射性谷氨酸或半胱氨酸。这些发现表明，肺细胞和红细胞中存在不同的细胞GSH调节机制。它们符合对胱氨酸-谷氨酸转运体以及转录和翻译事件的要求，以提高细胞GSH水平，以响应肺细胞中高氧或低水平的DEM。
We have previously found that exposure of pulmonary artery endothelial cells to hyperoxia or low concentrations of diethyl maleate (DEM) results in elevation of both cellular glutathione (GSH) and uptakes of glutamate and cystine. The present study confirms that this elevation occurs for a variety of lung cells (bovine pulmonary artery endothelial and smooth muscle cells and rat lung fibroblast and epithelial-like cells) but not for human, rat, and chicken erythrocytes. In fact, human and rat erythrocyte GSH levels were reduced substantially at DEM concentrations from 0.05 to 0.5 mM, whereas the GSH level of chicken erythrocytes was almost totally eliminated by 0.05 mM DEM. Also, all erythrocytes failed to accumulate measurable amounts of radioactive glutamate or cystine. The findings suggest the presence of different mechanisms for the regulation of cellular GSH in lung cells from those of erythrocytes. They are consistent with a requirement for a cystine-glutamate transporter and transcriptional and translational events for the elevation of cellular GSH in response to hyperoxia or low levels of DEM in the lung cells.