Direct analysis in real time mass spectrometry combined with single-drop liquid-liquid-liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice
Direct analysis in real time mass spectrometry combined with single-drop liquid-liquid-liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice
复制标题
实时质谱直接分析结合单滴液-液-液微萃取快速分析果汁中的多种植物激素
DOI：
10.1007/s00216-012-5728-x
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发表时间：
2012-06-01
影响因子：
4.3
通讯作者：
Liu, Huwei
中科院分区：
文献类型：
--
作者：
Bai, Yu;Zhang, Jialing;Liu, Huwei
本文建立了一种基于单滴液-液-液微萃取（SD-LLLME）与实时质谱直接分析（DART-MS）相结合的快速、简便、高效的多种植物激素快速测定方法。同时对吲哚-3-乙酸（IAA）、吲哚-3-丁酸（IBA）、茉莉酸（JA）、水杨酸（SA）、脱落酸（ABA）和赤霉素A(GA(3)) 6种植物激素进行分析，并对DART-MS和SD-LLLME的检测条件进行了系统优化。在线性（所有植物激素的R(2)值为0.991 ~ 0.996）、灵敏度（检测限为0.65 ~ 72 ng/mL）和重复性（RSD值为6.9 ~ 14%）方面均获得了满意的结果。并将该方法应用于三种果汁中内源植物激素的测定。检测不同浓度的植物激素，回收率满意，整个分析过程不超过30分钟。因此，SD-LLLME和DART-MS的结合被证明是一种适合和有效的方法，用于快速分析复杂基质中痕量浓度的目标。
A rapid, simple, and efficient method for the fast determination of multiple phytohormones was developed in this work, based on single-drop liquid-liquid-liquid microextraction (SD-LLLME) combined with direct analysis in real-time mass spectrometry (DART-MS). Six phytohormones-indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and gibberellin A(3) (GA(3))-were analyzed simultaneously using this method, and the conditions employed for DART-MS and SD-LLLME were optimized systematically. Satisfactory results were obtained in terms of linearity (R (2) values for all phytohormones were 0.991-0.996), sensitivity (limits of detection were 0.65-72 ng/mL), and repeatability (RSD values were 6.9-14%). In addition, the proposed method was applied to determine the endogenous phytohormones in three kinds of fruit juice. Different concentrations of phytohormones were detected with satisfactory recoveries, and the whole analytical procedure took no more than 30 min. Therefore, this combination of SD-LLLME and DART-MS was shown to be a suitable and effective approach for the fast analysis of targets present at trace level concentrations in complex matrices.