Nondestructive determination of leaf chlorophyll content in two flowering cherries using reflectance and absorptance spectra
Nondestructive determination of leaf chlorophyll content in two flowering cherries using reflectance and absorptance spectra
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DOI：
10.1007/s11355-009-0101-8
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发表时间：
2010-07-01
影响因子：
2
通讯作者：
Yoneda, Minoru
中科院分区：
文献类型：
--
作者：
Imanishi, Junichi;Nakayama, Aki;Yoneda, Minoru
叶片叶绿素定量是树木活力评价的关键技术。虽然许多研究已经进行了无损和现场光谱测定，它是合理的，以发展物种特异性叶绿素指数准确测定，因为叶片光谱可以独立于叶绿素含量，由于叶片表面和结构的差异物种之间。本研究的目的是建立最佳的反射率和吸收率指数，估算叶片叶绿素含量的金鱼草（Siebold ex Koidz.）H.大场湾jamasakura和Ceramaxyedoensis 'Somei-yoshino'的叶绿素指数，并通过与46个已发表的叶绿素指数和SPAD进行比较来检验它们的性能。对于96和100个叶片样品，使用具有叶夹附件的分光辐射计和SPAD-502叶绿素计进行测量，并且通过用N，N '-二甲基甲酰胺提取来测定叶绿素含量。通过对8种叶绿素指标的比较试验，系统地确定了最佳的叶片叶绿素指标。因此，我们证实，最佳的叶绿素指数表现优于任何公布的叶片叶绿素指数或SPAD，给RMSE是大约两倍的SPAD，并发现，新提出的指数类型的差异和比率组合类型可能是一个有用的形式的叶绿素含量估计。我们还发现，吸收率指数取得了等效的结果，反射率指数，尽管假设吸收率测量是直接的，并具有更大的潜力。在公布的指数中，Datt的反射率比率指数[Datt B（1999）Int J Remote Sens 20（14）：2741-2759]和Rendanda等人的红边叶绿素指数[Rendanda V，Gitelson A，Schepers J（2009）J Plant Physiol 166：157-167]在估计两种开花樱桃的叶片叶绿素含量方面是有效的。
Leaf chlorophyll quantification is a key technique in tree vigor assessment. Although many studies have been conducted on nondestructive and in-field spectroscopic determination, it is reasonable to develop species-specific chlorophyll indices for accurate determination, because leaf spectra can vary independently of chlorophyll content due to leaf surface and structural differences among species. The present study aimed to develop optimal reflectance and absorptance indices for estimating the leaf chlorophyll content of Cerasus jamasakura (Siebold ex Koidz.) H. Ohba var. jamasakura and Cerasus x yedoensis 'Somei-yoshino,' and to examine their performance by comparing them with 46 published chlorophyll indices and SPAD. For 96 and 100 leaf samples, measurements were taken using a spectroradiometer with a leaf-clip attachment and a SPAD-502 chlorophyll meter, and chlorophyll content was determined by extraction with N,N'-dimethylformamide. The optimal leaf chlorophyll indices were then developed systematically by testing eight types of indices. As a result, we confirmed that the optimal chlorophyll indices performed better than any of the published leaf chlorophyll indices or SPAD, giving RMSEs that were approximately twice as good as those for SPAD, and found that the newly proposed index type-a difference and ratio combination type-may be a useful form of chlorophyll content estimation. We also found that absorptance indices achieved equivalent results to reflectance indices despite the hypothesis that absorptance measurement is direct and has more potential. Among the published indices, the reflectance ratio index of Datt [Datt B (1999) Int J Remote Sens 20(14):2741-2759] and the red edge chlorophyll index of Ciganda et al. [Ciganda V, Gitelson A, Schepers J (2009) J Plant Physiol 166:157-167] were effective at estimating the leaf chlorophyll contents of both flowering cherries.