Effects of Distal Mutations on Prolyl-Adenylate Formation of Escherichia coli Prolyl-tRNA Synthetase.
Effects of Distal Mutations on Prolyl-Adenylate Formation of Escherichia coli Prolyl-tRNA Synthetase.
复制标题
DOI：
10.1007/s10930-020-09910-3
复制
发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Hati S
中科院分区：
文献类型：
--
作者：
Zajac J;Anderson H;Adams L;Wangmo D;Suhail S;Almen A;Berns L;Coerber B;Dawson L;Hunger A;Jehn J;Johnson J;Plack N;Strasser S;Williams M;Bhattacharyya S;Hati S
酶在许多生物过程中起着重要作用。酶活性位点口袋中的氨基酸残基与底物直接接触，通常被认为对底物识别和催化至关重要。识别和理解这些“催化”残基如何帮助酶实现巨大的速率提高一直是过去几十年来许多结构和生物化学研究的焦点。最近的研究表明，酶本质上是动态的，远距离结构元件之间的动态耦合对于模块酶中的有效催化是必不可少的。因此，预期远端残基会对酶功能产生影响。然而，很少有研究调查的作用，远端残基的酶催化。在本研究中，远端残基突变的氨酰-tRNA合成酶，即脯氨酰-tRNA合成酶的催化功能的影响进行了研究。本研究表明，远端残基显着有助于模块化大肠杆菌脯氨酰-tRNA合成酶的催化，保持内在的蛋白质的灵活性。
Enzymes play important roles in many biological processes. Amino acid residues in the active site pocket of an enzyme, which are in direct contact with the substrate(s), are generally believed to be critical for substrate recognition and catalysis. Identifying and understanding how these “catalytic” residues help enzymes achieve enormous rate enhancement has been the focus of many structural and biochemical studies over the past several decades. Recent studies have shown that enzymes are intrinsically dynamic and dynamic coupling between distant structural elements is essential for effective catalysis in modular enzymes. Therefore, distal residues are expected to have impacts on enzyme function. However, few studies have investigated the role of distal residues on enzymatic catalysis. In the present study, effects of distal residue mutations on the catalytic function of an aminoacyl-tRNA synthetase, namely, prolyl-tRNA synthase, were investigated. The present study demonstrates that distal residues significantly contribute to catalysis of the modular Escherichia coli prolyl-tRNA synthetase by maintaining intrinsic protein flexibility.
登录
查看更多内容
影响因子：
2.9
作者：
Johnson, James M.;Sanford, Brianne L.;Strom, Alexander M.;Tadayon, Stephanie N.;Lehman, Brent P.;Zirbes, Arrianna M.;Bhattacharyya, Sudeep;Musier-Forsyth, Karin;Hati, Sanchita
通讯作者：
Hati, Sanchita
影响因子：
2.9
作者：
Sanford, Brianne;Cao, Bach;Hati, Sanchita
通讯作者：
Hati, Sanchita
影响因子：
11.4
作者：
Cusack, S;Yaremchuk, A;Tukalo, M
通讯作者：
Tukalo, M
影响因子：
4.8
作者：
Hati, Sanchita;Ziervogel, Brigitte;Musier-Forsyth, Karin
通讯作者：
Musier-Forsyth, Karin
DOI：
10.1093/bioinformatics/btq662
发表时间：
2011-02-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Benkert P;Biasini M;Schwede T
通讯作者：
Schwede T