Application of ferriferous oxide modified by chitosan in gene delivery.
Application of ferriferous oxide modified by chitosan in gene delivery.
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DOI：
10.1155/2012/920764
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发表时间：
2012
影响因子：
--
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Kuang Y;Yuan T;Zhang Z;Li M;Yang Y
仍然需要新的方法来改进传统的基因载体。在这里，我们探索用可降解聚合物修饰Fe3O4，增强永久磁场下的基因传递和靶传递。采用可控化学共沉淀法合成了壳聚糖（CTS）和聚乙二醇（PEG）包覆的两种磁性Fe3O4纳米颗粒。质粒pEGFP被包封为已报道的基因。氧化亚铁配合物近似球形；CTS-Fe3O4和PEG-Fe3O4的表面电荷分别约为20 mv和0 mv。观察了CTS-Fe3O4纳米颗粒对DNA的控制释放。同时，通过体外细胞毒性试验验证了小于2 mM的Fe3O4浓度是安全的。永磁场的存在显著提高了转染效率。此外，体内实验还证实了磁性纳米颗粒的被动靶向性和安全性。这种新型的基因传递系统已被证明是未来在体内靶向表达和基因治疗所需的有效工具。
New approaches to improve the traditional gene carriers are still required. Here we explore Fe3O4 modified with degradable polymers that enhances gene delivery and target delivery using permanent magnetic field. Two magnetic Fe3O4 nanoparticles coated with chitosan (CTS) and polyethylene glycol (PEG) were synthesized by means of controlled chemical coprecipitation. Plasmid pEGFP was encapsulated as a reported gene. The ferriferous oxide complexes were approximately spherical; surface charge of CTS-Fe3O4 and PEG-Fe3O4 was about 20 mv and 0 mv, respectively. The controlled release of DNA from the CTS-Fe3O4 nanoparticles was observed. Concurrently, a desired Fe3O4 concentration of less than 2 mM was verified as safe by means of a cytotoxicity test in vitro. Presence of the permanent magnetic field significantly increased the transfection efficiency. Furthermore, the passive target property and safety of magnetic nanoparticles were also demonstrated in an in vivo test. The novel gene delivery system was proved to be an effective tool required for future target expression and gene therapy in vivo.