CK2-dependent C-terminal phosphorylation at T300 directs the nuclear transport of TSPY protein
CK2-dependent C-terminal phosphorylation at T300 directs the nuclear transport of TSPY protein
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DOI：
10.1016/j.bbrc.2005.12.190
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发表时间：
2006-03-10
影响因子：
3.1
通讯作者：
Arnemarm, J
中科院分区：
文献类型：
--
作者：
Krick, R;Aschrafi, A;Arnemarm, J
TSPY (testis-specific protein, Upsilon-encoded) is a member of the greater SET/NAP family of molecules with various functions, e.g., in chromatin remodeling, regulation of gene expression, and has been implicated to play a role in the malignant development of gonadoblastoma, testicular and prostate cancer. Here we demonstrate that the C-terminus has a functional role for the nucleo-cytoplasniatic shuttling of the TSPY protein. Using various combinations of in vitro mutagenesis and enhanced green fluorescent protein reporter gene-expression experiments we were able to show that while the deletion of C-terminus leads to a decreased stability and enhanced degradation of the protein, the selective imitation of a C-terminal CK2 phosphorylation site (T-300) prevents the TSPY protein from entering the nucleus. We conclude that phosphorylation of the (T-300) residue is a necessary and functional prerequisite for TSPY's transport into the nucleus reminding of comparable data from it related Drosophila molecule, NAP1. (c) 2006 Elsevier Inc. All rights reserved.
