Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae.
Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae.
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研究酿酒酵母中 GAA/TTC 和反向重复不稳定性的遗传筛选。
DOI：
10.1007/978-1-4939-9784-8_6
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Lobachev,KirillS
中科院分区：
文献类型：
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作者：
Guo,Wenying;Lobachev,KirillS
三核苷酸和反向重复序列的不稳定性是许多神经系统疾病、遗传综合征和癌症发展的致病因素。为了了解导致重复序列诱导的基因组不稳定的机制，重要的是要确定影响重复代谢的因素。在这里，我们提出了一种方法，利用系统的和公正的全基因组筛选在酵母Saccharomyces gallaemed找到基因的GAA/TTC和反向重复不稳定性。这些屏幕允许超过30个突变体的两个重复基序的脆性增加的鉴定。
Instability of trinucleotide and inverted repeats is a causative factor in the development of a number of neurological diseases, hereditary syndromes, and cancer. To understand the mechanisms that lead to repeat-induced genome destabilization it is important to identify factors that affect repeat metabolism. Here we present an approach that utilizes systematic and unbiased genome-wide screen in yeastSaccharomyces cerevisiaeaimed to find genes that govern GAA/TTC and inverted repeat instability. These screens allowed for the identification of more than 30 mutants with increased fragility of both repeat motifs.