Effect of fasting and acute ethanol administration on the energy state of in vivo liver as measured by 31P-NMR spectroscopy.
Effect of fasting and acute ethanol administration on the energy state of in vivo liver as measured by 31P-NMR spectroscopy.
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通过 31P-NMR 波谱测量禁食和急性乙醇给药对体内肝脏能量状态的影响。
DOI：
10.1016/0167-4889(86)90033-9
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Radda,GK
中科院分区：
文献类型：
--
作者：
Cunningham,CC;Malloy,CR;Radda,GK
应用~（31）P核磁共振波谱技术，研究了禁食48 h、乙醇或2，4-二硝基苯酚对肝脏含磷代谢产物的影响。将这些测量与在NMR测量完成后立即冷冻夹闭的肝脏中代谢物浓度的测定相结合。给药亚致死量的二硝基苯酚显著降低ATP和增加体内肝脏中的Pi浓度，如NMR衍生的[Pi][ATP]比率增加2.7倍所示。向进食动物给予乙醇使NMR衍生的[Pi][ATP]比率增加27%;相反，向禁食动物给予相同量的乙醇使NMR衍生的[Pi][ATP]比率降低30%。NMR可见的Pi和ADP分别占总Pi和ADP的约50%和15%。从NMR可见PI和ADP计算的磷酸化电位比从冷冻夹紧组织中的代谢物浓度获得的磷酸化电位高一个数量级。来自NMR光谱分析或代谢物浓度的磷酸化电位与肝脏[NAD+][NADH]比值之间无明显相关性。PI的化学位移表明，乙醇给药引起体内肝脏pH值降低0.1单位。肝脏游离[Mg 2+]在禁食动物中增加21%，但不受乙醇给药的影响。
The effects of 48 h fasting, administration of ethanol or 2, 4-dinitrophenol, on the phosphorus-containing metabolites in liver in vivo have been determined utilizing 31 P nuclear magnetic resonance spectroscopy. These measurements were combined with determinations of metabolite concentrations in livers which were freeze-clamped immediately after the NMR measurements were completed. Administration of sub-lethal amounts of dinitrophenol dramatically decreased ATP and increased P i concentrations in liver in vivo as indicated by a 2.7-fold increase in the NMR-derived [P i][ATP] ratio. Ethanol administration to fed animals increased the NMR-derived [P i][ATP] ratio 27%; in contrast, the same amount of ethanol administered to fasted animals decreased the NMR-derived [P i][ATP] ratio 30%. The NMR visible P i and ADP represent about 50% and 15% of the total P i and ADP, respectively. The phosphorylation potentials calculated from the NMR visible P i and ADP were an order of magnitude higher than those obtained from metabolite concentrations in freeze-clamped tissue. There was no apparent correlation between the phosphorylation potentials derived from either the NMR spectral analyses or from metabolite concentrations and the hepatic [NAD+][NADH] ratio. The chemical shift of P i indicated that ethanol administration elicited a decrease in pH of 0.1 unit in liver in vivo. Hepatic free [Mg 2+] was increased 21% in fasted animals, but was unaffected by ethanol administration.